| 摘要: |
| 在全缘马尾藻生殖细胞同步成熟的基础上，研究了生殖细胞的排放规律以及人工促排的条件。结果表明：（1）在温度（25±0.5）℃，光照强度（3000±100）lx，光照周期12h︰12h[光照（L）︰黑暗（D）]，盐度33±0.5，pH值8.2±0.2、静置暂养条件下，雄生殖托排放精子与雌生殖托排放卵细胞基本同步；雌生殖托排放卵细胞集中在夜间，为间断式排卵，完成整个排卵过程约需3d；雌生殖托的排卵量与其重量呈正相关，3d内排出卵细胞（14431±1288）个/g；卵细胞在排出后4h内完成受精，得到受精卵8358±734个/g；雌生殖托表面的“挂卵”量约为10000个/g，受精卵的脱落量与排卵量相关。（2）在光照周期0h：24h（L︰D）、pH值8.2培养条件的基础上，最适生殖细胞促排条件分别为温度31℃，盐度33±1，水流速度（6±1）cm/s，在该条件下得到的脱落受精卵量最多，所需促排及排放时间最短。在最适条件下，将雌、雄生殖托混合的重量比例改变为1︰6，受精率可提高至（94.4±0.3）%，相比暂养条件下的排卵过程，排卵提前4d，排卵周期缩短2d。 |
| 关键词: 全缘马尾藻 生殖细胞 排放规律 促排 |
| DOI：10.11693/hyhz20160600126 |
| 分类号: |
| 基金项目:广东省科技计划项目，2006B60501016号，2009B080701048号。 |
附件 |
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| REGULATION OF GAMETES RELEASE AND ARTIFICIAL DECORPORATION CONDITION FOR SARGASSUM INTEGERRIMUM |
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OU Ze-Kui, LIU Dong-Chao, XIE En-Yi, WU Qi-Fan
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Guangdong Ocean University, Zhanjiang 524088, China
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| Abstract: |
| We investigated the regulation of gametes release and the artificial decorporation condition based on the gametes synchronous maturity of Sargassum integerrimum. Gametes release regulation was observed under temporary rearing condition (25±0.5℃, illumination intensity (3000±100)lx, photoperiod 12h:12h, salinity 33, pH 8.2±0.2, and water velocity 0cm/s). The sperm discharged from male receptacles synchronized with eggs discharged from female receptacles. The female receptacles discharged eggs in dark time interval in 3 days discontinuously. The amount of released eggs is positively associated with weights of female receptacles. After 3 days, the rate of total released eggs was 14431±1288 eggs/g. Eggs remained active in 4h after being discharged and that of zygote was (8358±734) zygotes/g in the end. Some 10000 eggs or exutive eggs per gram adhered to the surface of female receptacles under stirless seawater condition. The amount of exutive eggs was positively associated with the amount of released eggs. Meanwhile, under the conditions of photoperiod 0h:24h(L:D), and pH 8.2, the optimal condition for gamete release was determined:temperature 31℃, salinity 33±1, and water velocity (6±1)cm/s. The most exutive zygotes would be obtained and the least decorporation time and release time would be cost under optimal condition above. The fertilization rate can be elevated to (94.4±0.3)% when weight rate of female/male receptacles was 1 to 6 under the optimum condition, and the decorporation time could be shortened by 4 days and the gametes release time be shortened by 2 days compared to those in release process under temporary rearing conditions. |
| Key words: Sargassum integerrimum gametes release regulation artificial decorporation |
