| 摘要: |
| 为筛选适合添加于草鱼（Ctenopharyngodon idellus）饲料的植物源免疫增强剂，本试验以白芍（Radix paeoniae Alba）、杜仲（Eucommia ulmoides）和知母（Rhizoma anemarrhenae）三种允饲植物配制的复方制剂A、B和C为研究对象，开展其对草鱼非特异性免疫功能的比较研究。试验将二龄草鱼随机分成6个试验组和1个对照组，试验组和对照组按鱼体重量的3%每天分别投喂添加复方制剂的饲料和空白饲料。试验第7、14、21、28天分别采集试验草鱼血液，用于检测血液细胞组分、血清溶菌酶、血清SOD；试验第29天，对各组试验草鱼进行腹腔注射嗜水气单胞菌活菌，记录攻毒后14天内各组草鱼的存活率。结果表明，与对照组相比，试验第14天时，以2%和4%剂量添加的复方制剂A分别显著提高草鱼白细胞吞噬活性128%和143%，分别提高草鱼血清溶菌酶活性69%和85%，分别提高草鱼血清SOD活性61%和55%；连用28天对草鱼的保护率分别达到49%和55%，复方制剂A对草鱼非特异性免疫增强效果优于复方制剂B和C。 |
| 关键词: 允饲植物 复方制剂 草鱼 免疫增强 存活率 |
| DOI：10.11693/hyhz20161200273 |
| 分类号:Q176;R285;S942.5 |
| 基金项目:广东省博士后科研基金专项资助，B150787号。 |
附件 |
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| IMMUNE ENHANCEMENT FOR GRASS CARP CTENOPHARYNGODON IDELLUS WITH HERBAL ENRICHED FEED |
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HUANG Jin-Lu1,2, LI Ai-Hua1, QIAN Xue-Qiao2, TAN Xiao-Chen2, WANG Zheng-Kai2
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1.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;2.Animal Husbandry and Fisheries Research Center of Guangdong Haid Group Co., Limited, Guangzhou 511400, China
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| Abstract: |
| To screen feeding plant candidates suitable for immune enhancement of grass carp Ctenopharyngodon idellus, three herbs, Radix paeoniae Alba (A), Eucommia ulmoides (B), and Rhizoma anemarrhenae (C) were tested. They were water extracted separately and added at different ratios into compound feed. Two-year-old grass carps were randomly divided into 6 groups against a control, and they were fed with A-, B-, and C-enriched feed separately, while the control was feed without any additive, at 3% of body weight per day. Experiment lasted for 43 days, during which blood samples were collected on Days 7, 14, 21, and 28, for the detections of blood cell components, serum lysozyme, and serum SOD, and then the experimental fish were challenged by Aeromonas hydrophila via intraperitoneal injection on Day 29. The results show that the phagocytic activity of leukocytes, lysozyme activity, and SOD activity of the fish fed on extract A enriched feed for 7-14 days were significantly increased (P<0.05), and their survival rate 14 days post-challenge reached about a half. In addition, among the three herbs, A was shown the best performance in the immune enhancement than B and C. In the future, searching for more good natural plants to prove aquaculture performance shall be encouraged. |
| Key words: feeding plant preparation grass carp Ctenopharyngodon idellus immune enhancement survival rate |
