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三角帆蚌(Hyriopsis cumingii)叉头蛋白2(foxl2)基因和性别决定框14(sox14)基因克隆与表达分析
叶容晖1, 任海波2
1.金华职业技术学院 金华 321007;2.宁波海洋研究院 宁波 315012
摘要:
三角帆蚌(Hyriopsis cumingii)生殖发育研究相对较少,相关基因信息缺乏。本文以三角帆蚌卵巢和精巢为研究对象,对转录组测序获得的叉头蛋白2(foxl2)基因和性别决定框14(sox14)基因保守区进行克隆和表达分析。定量PCR显示foxl2主要在卵巢中表达,而sox14主要在精巢中表达。在不同发育时期,foxl2和sox14在受精卵和卵裂期基本不表达,从原肠期开始表达,钩介幼虫期表达量比较高。原位杂交染色证明foxl2基因主要表达在卵巢的间质细胞和颗粒细胞中,sox14在精巢的间质细胞和一些精原细胞中表达。三角帆蚌foxl2的表达和脊椎动物有类似的模式,foxl2可能在河蚌卵巢发育中起到重要作用,可以作为卵巢的一个标志分子;Sox14可能对精巢发育和功能维持有重要作用,可以作为精巢的一个标记分子。Foxl2和sox14在胚胎发育中也会发挥一定的功能。本文结果丰富了河蚌的基因资源,可为三角帆蚌的生殖与发育生物学研究提供基础数据。
关键词:  三角帆蚌  foxl2基因  sox14基因  生殖腺
DOI:10.11693/hyhz20170600165
分类号:Q731
基金项目:浙江省自然科学基金资助项目,LY14C190010号。
MOLECULAR CLONING AND GENE EXPRESSION OF FOXL2 AND SOX14 GENE FROM HYRIOPSIS CUMINGII
YE Rong-Hui1, REN Hai-Bo2
1.Jinhua Polytechnic, Jinhua 321007, China;2.Ningbo Institute of Oceanography, Ningbo 315012, China
Abstract:
The study on Sexual development and gene resources is relatively lacking in Hyriopsis cumingii. Here we analyzed the expression of foxl2 and sox14 gene in the gonad. The results showed that foxl2 was mainly expressed in the ovary, but, minor expression was observed in other tissues selected in this study. While sox14 was mainly expressed in the testis, but minor expression was observed in other tissues. During different developmental stages, the foxl2 and sox14 were barely expressed in fertilized eggs and cleavage stage, but expressed obviously after gastrulae stage. In situ hybridization indicated that the foxl2 was mainly expressed in the follicle cells and interstitial cells. While sox14 was mainly expressed in interstitial cells and spermatid. The results also indicated that foxl2 and sox14 gene showed a similar expression pattern to that of vertebrates. Foxl2 gene has the essential function in ovary development and could act as a marker of the ovary in H. cumingii. Meanwhile, soxl4 gene has the essential function in testis development and could act as a marker of testis. Foxl2 and sox14 also play a role during the embryo development. This study enriched gene resources and provided fundamental data for sexual and developmental biology research of H. cumingii.
Key words:  Hyriopsis cumingii  foxl2 gene  sox14 gene  gonad
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