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引用本文:李笑,曲艺,张倩倩,张天宇,曹瑞文,赵建民.海水酸化和热应激对日本鼓虾氧化应激和能量代谢的影响.海洋与湖沼,2020,51(6):1412-1421.
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海水酸化和热应激对日本鼓虾氧化应激和能量代谢的影响
李笑1,2,3, 曲艺1,2,3, 张倩倩1,2, 张天宇1,2,3, 曹瑞文1,2,3, 赵建民1,2,4
1.中国科学院烟台海岸带研究所 牟平海岸带环境综合试验站 烟台 264117;2.中国科学院烟台海岸带研究所 海岸带生物资源高效利用研究与发展中心 烟台 264003;3.中国科学院大学 北京 100049;4.中国科学院海洋大科学研究中心 青岛 266071
摘要:
伴随着全球气候变化的日益加剧,过量CO2排放所导致的海洋酸化和暖化现象已引起广泛关注。本研究设置了两个pH水平(pH 8.1和pH 7.6)和两个温度梯度(20.0和23.0℃),以探讨海水酸化和温度升高对日本鼓虾(Alpheus japonicus Miers)氧化应激和能量代谢过程的影响。结果发现,海水酸化、热应激以及海水酸化和热应激复合胁迫能够诱导日本鼓虾产生不同程度的氧化应激现象,且海水酸化和热应激对过氧化氢酶(Catalase,CAT)活性、还原型谷胱甘肽(Glutathione,GSH)含量和还原型谷胱甘肽与氧化型谷胱甘肽比值(Glutathione/Oxidized Glutathione,GSH/GSSG)有交互作用;其中,海水酸化和热应激复合暴露导致鼓虾GSH含量较对照组水平降低了66.0%,GSH/GSSG比值降低为对照组水平的20.8%,而脂质过氧化水平则较对照组显著增加了51.4%。此外,海水酸化和热应激复合暴露能够导致日本鼓虾己糖激酶(Hexokinase,HK)活性的显著增强和蛋白质含量的显著降低,提示鼓虾通过调节糖酵解过程和能量储备来满足机体的能量代谢需求。上述结果表明,短期海水酸化和热应激暴露能够影响日本鼓虾的抗氧化防御体系、能量代谢过程以及能量储备能力,长期持续暴露可能会对其种群维持构成潜在威胁。
关键词:  日本鼓虾  海水酸化  热应激  氧化应激  能量代谢
DOI:10.11693/hyhz20200200042
分类号:S968.2
基金项目:国家重点研发计划,2018YFC1406503号。
EFFECTS OF SEAWATER ACIDIFICATION AND THERMAL STERSS ON THE ANTIOXIDANT RESPONSES AND ENERGY METABOLISM OF ALPHEUS JAPONICUS MIERS
LI Xiao1,2,3, QU Yi1,2,3, ZHANG Qian-Qian1,2, ZHANG Tian-Yu1,2,3, CAO Rui-Wen1,2,3, ZHAO Jian-Min1,2,4
1.Muping Coastal Environmental Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264117, China;2.Research and Development Center for Efficient Utilization of Coastal Bioresources, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;3.University of Chinese Academy of Sciences, Beijing 100049, China;4.Center for Ocean Mega-science, Chinese Academy of Sciences, Qingdao 266071, China
Abstract:
As a consequence of global climate change in the last two centuries, ocean acidification resulted in warming waters. In this study, we explored the physiological responses of snapping shrimp Alpheus japonicus Miers, an abundant and broadly distributed species of marine crustacean, to modern (400ppm) and future ambient CO2 (2000ppm) seawater acidic levels at two temperatures (20, 23℃) in experiment. Results show that the elevated acidic and thermal stresses led to oxidative stress, and acidic and thermal stresses had interactive effects on the catalase (CAT) activity, glutathione (GSH) content and the ratio of glutathione (GSH) to oxidized glutathione (GSSG), GSH content and the GSH/GSSG ratio of the shrimp were significantly inhibited by 66.0% and 20.8%, respectively, while the LPO level increased by 51.4% in contrast to the control's. Moreover, the HK activity was increased and PROT content reduced, showing disturbance in glycolysis and energy reserves. In other words, the energy metabolism strategy adopted by A. japonicus would be not sustainable in the long term. In addition, the short-term seawater acidification and elevated thermal stress in the experiment triggered oxidative stress and disturbance in energy metabolism of A. japonicus. Therefore, global warming and ocean acidification would affect the population replenishment of the species, as well as other ocean creatures in the future.
Key words:  Alpheus japonicus Miers  seawater acidification  thermal stress  antioxidant response  energy metabolism
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