引用本文:	赵东会,赵丽达,尤宏,秦松,王寅初,焦绪栋.耐高温球等鞭金藻(Isochrysis galbana)藻种的选育与饵料特性初步分析.海洋与湖沼,2021,52(1):206-212.

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*耐高温球等鞭金藻(Isochrysis galbana)藻种的选育与饵料特性初步分析*
赵东会^1,2, 赵丽达³, 尤宏¹, 秦松^2,4, 王寅初^2,4, 焦绪栋^2,4
1.哈尔滨工业大学(威海)海洋科学与技术学院威海 264209;2.中国科学院烟台海岸带研究所烟台 264003;3.南阳师范学院生命科学与技术学院南阳 473061;4.中国科学院海洋大科学研究中心青岛 266071

摘要:

通过显微操作技术从海水饵料微藻培育池中分离出4株球等鞭金藻（Isochrysis galbana）藻株，测试其在高水温（33℃，40℃）下的生长性能；对藻株的颗粒大小和粗蛋白含量进行了测定；筛选出一株生长速度较快的球等鞭金藻PHY7004，优化了其培养条件；分析了单环刺螠幼苗对其的摄食能力。结果表明，分离出的4株球等鞭金藻可在水温33℃下生长，其中PHY7004生长速率最快；分离出的各藻株细胞的平均直径为5.5—7.5 μm；耐受33℃的4株藻株平均鲜重蛋白质含量为34.29 mg/L，其中PHY7003蛋白质含量最高，为41.80 mg/L；优化后的最适培养条件为pH=7，N/P=14：1；单环刺螠幼苗对PHY7002的摄食强度较高。本实验为耐高温饵料藻种的筛选提供参考，也可为单环刺螠育苗所用的饵料微藻的选择提供依据。

关键词: 球等鞭金藻耐高温饵料微藻单环刺螠

DOI：10.11693/hyhz20200300091

分类号:X55;Q148

基金项目:中国科学院青年创新促进会人才计划项目；烟台市海洋经济创新发展示范城市项目，YHCX-SW-L-201703号；福州市海洋经济创新发展示范城市项目，FZHJ04号；莱州市科技计划项目，201801—201912号。

附件

BREEDING AND FEEDING CHARACTERISTICS OF HIGH-TEMPERATURE-RESISTANT STRAINS OF ISOCHRYSIS GALBANA

ZHAO Dong-Hui^1,2, ZHAO Li-Da³, YOU Hong¹, QIN Song^2,4, WANG Yin-Chu^2,4, JIAO Xu-Dong^2,4

1.School of Marine Science and Technology, Harbin Institute of Technology(Weihai), Weihai 264209, China;2.Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;3.School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China;4.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China

Abstract:

Four strains of Isochrysis galbana, named PHY7001-PHY7004, were isolated in microscope and tested for their growth characteristics in high temperatures (33℃, 40℃). The cell size and protein content of the strains were determined and the medium of the one with the fastest growth rate (PHY7004) was optimized. In addition, we conducted feeding experiment of Urechis unicinctus seedlings. Results show that the four strains could grow best at 33℃, of which the growth rate of PHY7004 was the biggest. The average diameter of cell size was 5.5-7.5 mm, the average fresh-weight protein content of the 4 strains was 34.29 mg/L, of which the protein content of PHY7003 was the highest:41.80 mg/L. The optimum culture conditions for the fast-growing PHY7004 were pH=7 and N/P=14:1; and U. unicinctus seedling of PHY7002 feeding intensity was the highest. This study provides a reference for screening of high-temperature-resistant bait, and a basis for the bait selection of U. unicinctus seedling.

Key words: Isochrysis galbana hyperthermia-resistant bait microalgae Urechis unicinctus