| 摘要: |
| 钙（Ca2+）作为一种必需的信号分子，在植物的生长发育和非生物胁迫调节中起着至关重要的作用。本实验采用BGISEQ平台进行高通量测序，获得浒苔外源CaCl2添加（UpCa）和高温对照（UpHT）处理下相关的转录组数据，分析了在高温（35℃）下外源CaCl2的添加转录组的变化。结果显示，根据UpHT和UpCa处理分析共获得36625条基因；与UpHT相比，在UpCa下鉴定出7513个差异表达的基因，包括6002个上调基因，1151个下调基因，对差异基因进行了GO富集和KEGG富集分析。对膜转运、植物信号转导和环境适应性相关的差异基因进行KEGG富集分析，主要富集在内吞（Endocytosis）、植物病原菌互作（Plant-pathogen interaction）、丝裂原激活的蛋白激酶信号通路（MAPK signaling pathway-plant）、植物激素的信号传递（Plant hormone signal transduction）、ABC转运（ABC transporters）和磷脂酰肌醇信号系统（Phosphatidylinositol signaling system）等代谢通路上。植物激素信号转导途径中，细胞分裂素、脱落酸、油菜素内酯和乙烯信号转导途径增强。抗氧化酶中FeSOD、谷胱甘肽过氧化物酶、谷胱甘肽S转移酶和抗坏血酸过氧化物酶等基因表达上调，下调的基因主要是热激蛋白。Ca2+信号组分基因钙结合蛋白、钙调素、钙/钙调素依赖的蛋白激酶以及钙/钙调蛋白依赖性3'，5'环核苷酸磷酸二酯酶的基因表达上调，丝裂原活化蛋白激酶激酶激酶和磷脂酰肌醇信号相关的基因也差异性上调。本研究系统阐述了植物信号转导、抗氧化酶、MAPK信号系统以及Ca2+信号组分基因在外源CaCl2对浒苔高温压力的调节中的重要作用，可为进一步阐明Ca2+信号对高温的调节适应机制提供理论基础。 |
| 关键词: 浒苔(Ulva prolifera) 外源氯化钙 转录组 高温 |
| DOI：10.11693/hyhz20201000297 |
| 分类号:Q523.8;Q789;S917 |
| 基金项目:浙江省省属高校基本科研业务费项目，2019J00049号；浙江省自然科学基金项目，LY20D060006号；宁波大学海洋学院联合实验室开放基金项目，2019—2021；浙江海洋大学博士启动基金项目，Q1701号；浙江省一流学科开放基金项目，2019—2021。 |
附件 |
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| COMPARATIVE TRANSCRIPTOME STUDY OF ULVA PROLIFERA TO CALCIUM CHLORIDE TREATMENT UNDER HIGH TEMPERATURE STRESS |
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TANG Xiao-Wen1, FAN Mei-Hua1, WANG Chao-Feng1, LIAO Zhi1, LI Peng1, XU Nian-Jun2,3,4,5, WANG Jian-Xin1
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1.Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316022, China;2.National Engineering Research Laboratory of marine biotechnology and Engineering, Ningbo University, Ningbo 315832, China;3.Key Laboratory of Applied Marine Biotechnology (Ningbo University), Ministry of Education, Ningbo 315832, China;4.Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315832, China;5.Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo 315832, China
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| Abstract: |
| As a necessary signal molecule, calcium (Ca2+) plays an important role in plant growth and abiotic stress regulation. The transcriptome variation of exogenous CaCl2 addition in U. Prolifera at a high temperature (35℃) was analyzed in experiment using BGISEQ platform. The high temperature with CaCl2 addition was the treatment group (denoted UpCa), and high temperature was the control group (UpHT). Results show that 36625 genes were obtained, of which 7513 differentially expressed genes were identified, including 6002 up-regulated genes and 1151 down-regulated genes. The DEGs related to membrane transport, plant signal transduction and environmental adaptability were analyzed. They were mainly enriched in endocytosis, plant pathogen interaction, plant hormone signal transduction, mitogen activated protein kinase (MAPK signaling pathway plant), ABC transporters, and phosphatidylinositol signaling system. In the metabolic pathway of plant signaling transduction system, cytokinin, abscisic acid, brassinolide, and ethylene signal transduction pathways were enhanced. The expressions of FeSOD, GSHPx, glutathione-s-transferase, and ascorbic acid peroxidase were up-regulated, and the down regulated genes were heat shock protein. The expressions of calcium binding protein, calcium/calmodulin dependent protein kinase, calmodulin, and calcium/calmodulin dependent 3',5'-cyclic nucleotide phosphodiesterase were up-regulated. The mitogen activated protein kinase kinase kinase (MAPKKK) and phosphatidylinositol signal related genes were also up-regulated. In this study, the important roles of plant signal transduction, antioxidant enzyme, MAPK signal system, and Ca2+ signal component genes in exogenous CaCl2 regulation of high temperature pressure in U. prolifera were systematically reviewed. These results will provide a basis for further elucidating the mechanism of Ca2+ signal regulation and adaptation to high temperature. |
| Key words: Ulva prolifera exogenous CaCl2 transcriptome high temperature |
