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引用本文:吴波,张顺,张成,母昌考,徐善良,王丹丽.外源神经递质5-羟色胺(5-HT)和多巴胺(DA)调控三疣梭子蟹争斗行为的初步研究.海洋与湖沼,2022,53(1):168-175.
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外源神经递质5-羟色胺(5-HT)和多巴胺(DA)调控三疣梭子蟹争斗行为的初步研究
吴波1, 张顺1, 张成1, 母昌考1, 徐善良1,2, 王丹丽1
1.宁波大学海洋学院 浙江宁波 315211;2.海洋生物技术教育部重点实验室 浙江宁波 315211
摘要:
影响甲壳类动物争斗行为的因素包括性别差异、体型和生殖状态等,神经递质对甲壳动物的争斗行为也具有调控作用。为探索三疣梭子蟹在养殖和繁殖过程中由于争斗行为受损或死亡的行为机制,采用双抗体夹心法测定争斗前后三疣梭子蟹血淋巴中5-HT和DA的含量,并分析了争斗前后肌肉、胸神经节、肝胰腺三种组织中5-HT和DA受体基因表达量变化。同时,通过注射外源5-HT和DA,记录并分析了三疣梭子蟹争斗行为差异以及三种组织中5-HT和DA受体基因表达量变化。结果表明:(1)争斗后三疣梭子蟹血淋巴中5-HT含量显著升高,DA含量显著降低(P<0.05);(2)注射10–6 mol 5-HT能显著减少三疣梭子蟹的争斗行为发生次数以及争斗持续时间(P<0.05),而注射10–6 mol DA却能够显著增加三疣梭子蟹的争斗行为发生次数和争斗持续时间(P<0.05);(3)争斗前后5-HT和DA受体在肌肉和胸神经节中表达量发生显著变化(P<0.05),且不同受体在相同组织中未呈现相同的变化趋势;(4)注射外源DA能够调节5-HT1B、5-HT2B、5-HT7受体的表达水平(P<0.05),外源5-HT也能调节DA1ADA2受体的表达水平(P<0.05)。综上所述,外源5-HT对三疣梭子蟹争斗行为起抑制作用,而DA对三疣梭子蟹争斗行为起促进作用。同时,外源5-HT和DA也会引起相关受体表达量变化,且它们之间存在相互作用。
关键词:  三疣梭子蟹  争斗行为  5-HT/DA  5-HT/DA受体
DOI:10.11693/hyhz20210600132
分类号:S967;Q958.12
基金项目:浙江省重大科技专项,2019C02057号;国家自然科学基金项目,42076087号。
附件
PRELIMINARY STUDY ON THE REGULATION OF AGONISTIC BEHAVIOR OF PORTUNUS TRITUBERCULATUS BY EXOGENOUS 5-HT AND DA
WU Bo1, ZHANG Shun1, ZHANG Cheng1, MU Chang-Kao1, XU Shan-Liang1,2, WANG Dan-Li1
1.School of Marine Science, Ningbo University, Ningbo 315211, China;2.Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China
Abstract:
To explore the behavioral mechanism of damage or death of Portunus trituberculatus due to agonistic behavior during culture and reproduction, contents of 5-HT and DA in hemolymph of P. trituberculatus before and after fight were determined by double antibody sandwich method, and the changes of 5-HT and DA receptor gene expression in muscle, thoracic ganglion, and hepatopancreas before and after fight were analyzed. Meanwhile, the difference of agonistic behavior and the changes of 5-HT and DA receptor gene expression in the three tissues were recorded and analyzed by injecting exogenous 5-HT and DA. Results show that, after fighting, the content of 5-HT in hemolymph of P. trituberculatus increased significantly, while the content of DA decreased significantly (P<0.05). Injection of 10–6-mol 5-HT could significantly reduce the frequency and duration of agonistic behavior (P<0.05), while injection of 10–6-mol DA could significantly increase the frequency and duration of agonistic behavior of P. trituberculatus (P<0.05). The expression of 5-HT and DA receptor in muscle and thoracic ganglion changed significantly before and after fight, and different receptors did not show the same trend in the same tissue. Exogenous 5-HT could also regulate the expression of DA1A and DA2 receptors, and exogenous DA could regulate the expression of 5-HT1B, 5-HT2B, and 5-HT7 receptors. Therefore, 5-HT can inhibit the agonistic behavior of P. trituberculatus, while DA can promote the agonistic behavior. In addition, 5-HT and DA receptor genes can participate in the regulation of agonistic behavior of P. trituberculatus through 5-HT/DA, interactively.
Key words:  Portunus trituberculatus  agonistic behavior  5-HT/DA  5-HT/DA receptor
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