引用本文:	林卓如,耿慧霞,唐文娇,于仁成.麻痹性贝毒在毛蚶体内的转化过程研究.海洋与湖沼,2022,53(5):1131-1142.

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麻痹性贝毒在毛蚶体内的转化过程研究
林卓如^1,2, 耿慧霞¹, 唐文娇^1,3, 于仁成^1,4,2,5
1.中国科学院海洋研究所海洋生态与环境科学重点实验室山东青岛 266071;2.中国科学院大学北京 100049;3.中国海洋大学海洋生命学院山东青岛 266003;4.青岛海洋科学与技术试点国家实验室海洋生态与环境科学功能实验室山东青岛 266071;5.中国科学院海洋大科学研究中心山东青岛 266071

摘要:

麻痹性贝毒能够在贝类体内累积,威胁海产品消费者健康。在以往调查中,多次在毛蚶(Scapharca subcrenata)体内发现高含量的麻痹性贝毒,但对于毛蚶体内麻痹性贝毒的转化过程及其食品安全风险还缺乏认识。通过室内模拟实验,选择太平洋亚历山大藻(Alexandrium pacificum)和链状裸甲藻(Gymnodinium catenatum)作为产毒藻种,研究了两种有毒藻种所产麻痹性贝毒在毛蚶体内的转化过程。结果表明,毛蚶体内主要出现了三种麻痹性贝毒转化过程,一是R1位羟基的还原反应,二是N-磺酰氨甲酰基类毒素R4位磺酸基团的水解反应,三是含羟基苯甲酸(hydroxybenzoate)基团的链状裸甲藻毒素在R4位的水解反应。毛蚶体内麻痹性贝毒的生物转化过程复杂,对毛蚶毒性的影响具有一定的不确定性,未来仍需要进一步深化毛蚶体内毒素累积、代谢、转化过程的研究,同时加强对毛蚶体内毒素含量的全面监测,防范毛蚶可能导致的麻痹性贝毒中毒风险。

关键词: 麻痹性贝毒毛蚶太平洋亚历山大藻链状裸甲藻链状裸甲藻毒素毒素转化

DOI：10.11693/hyhz20220100024

分类号:X174

基金项目:国家重点研发计划,2017YFC1600701号;科技基础资源调查专项,2018FY100202号;国家自然科学基金委员会联合基金项目,U20A20104号。

附件

BIOTRANSFORMATION OF PARALYTIC SHELLFISH TOXINS IN BLOOD CLAM SCAPHARCA SUBCRENATA

LIN Zhuo-Ru^1,2, GENG Hui-Xia¹, TANG Wen-Jiao^1,3, YU Ren-Cheng^1,4,2,5

1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.College of Marine Life Science, Ocean University of China, Qingdao 266003, China;4.Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071, China;5.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China

Abstract:

The accumulation of paralytic shellfish toxins in marine bivalves pose severe threats to human health.Scapharca subcrenata may contain high concentrations of paralytic shellfish toxins,yet toxin biotransformation in S.subcrenata and its effect on the toxicity of the bivalve remain largely unknown.Therefore,biotransformation of paralytic shellfish toxins in S.subcrenata were investigated by feeding S.subcrenata with two toxin-producing dinoflagellates,Alexandrium pacificum and Gymnodinium catenatum.Reduction of hydroxyl at R1,hydrolysis of sulfocarbamoyl group at R4 in N-sulfocarbamoyl toxins,and hydrolysis of hydroxybenzoate group at R4 in Gymnodinium catenatum toxins are the main biotransformation processes in S.subcrenata.The complex biotransformation of paralytic shellfish toxins in S.subcrenata contributes to the uncertainty of toxicity.To reduce risks associated with paralytic shellfish poisoning,further investigations on accumulation,transformation,and elimination of paralytic shellfish toxins in S.subcrenata should be carried out,and more efforts are needed to monitor toxins in S.subcrenata.

Key words: paralytic shellfish toxins Scapharca subcrenata Alexandrium pacificum Gymnodinium catenatum Gymnodinium catenatum toxins toxin biotransformation