| 摘要: |
| 为研究低压静电场对半滑舌鳎(Cynoglossus semilaevis)在贮藏过程中微生物群落特征, 采用高通量测序技术对贮藏至30 d半滑舌鳎的微生物群落碱基信息测序, 产生的碱基序列通过OTU聚类、多样性分析、相关性分析等手段, 探讨影响半滑舌鳎保鲜效果的微生物群落组成和发育信息。研究不同低压静电场(2 000、2 500 V/m)对半滑舌鳎贮藏期间微生物群落组成的影响。结果表明: 半滑舌鳎有效序列范围为76 735~103 583, 平均长度为428.39~429.17 bp, OTU数目在45~218。贮藏期间样品的微生物多样性和相对丰度均有所下降。在门水平上共鉴定出四种微生物菌门, 分别为变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidota)和其他。在属水平上鉴定出11种微生物菌属, 分别为不动杆菌属(Acinetobacter)、嗜冷杆菌属(Psychrobacter)、希瓦氏菌属(Shewanella)、环丝菌属(Brochothrix)、漫游菌属(Vagococcus)、发光杆菌属(Photobacterium)、Myroides和肉食杆菌属(Carnobacterium)、假单胞菌属(Pseudomonas)、无色杆菌属(Achromobacter)和其他。贮藏至30 d时, CK组优势腐败菌为环丝菌属, LVEF-1 (2 000 V/m)和LVEF-2 (2 500 V/m)组样品中环丝菌属相对丰度显著上升, 但是低于CK组, 且嗜冷杆菌属和希瓦氏菌属相对丰度仍然占据一定比例。由此推测LVEF能够抑制半滑舌鳎优势腐败菌属相对丰度的增加。研究结果为低压静电场保鲜贮藏半滑舌鳎微生物多样性变化提供了理论基础。 |
| 关键词: 半滑舌鳎(Cynoglossus semilaevis) 低压静电场 高通量测序 微生物多样性 |
| DOI：10.11693/hyhz20230600132 |
| 分类号:TS254; Q789 |
| 基金项目:水产品陆海联动冻藏冷链物流贮运与品质监控关键技术及装备研发项目;“十三五”国家重点研发计划重点专项,2019YFD0901604号 |
附件 |
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| ANALYSIS OF EFFECT OF LOW VOLTAGE ELECTROSTATIC FIELD ON THE PRESERVATION AND MICROBIAL COMMUNITY STRUCTURE OF CYNOGLOSSUS SEMILAEVIS |
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YANG Sha-Sha1, XIE Chao1, LIN Lin1, ZHENG Wei1, ZHU Ya-Meng1, ZHANG Hai-Ling2
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1.Zhejiang Provincial Key Laboratory of Health Risk Factors for Seafood, College of Food and Medicine, Zhejiang Ocean University, Zhoushan 316022, China;2.Zhoushan HSBC Cold Storage Logistics Development Co. Ltd., Zhoushan 316002, China
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| Abstract: |
| To investigate the effect of different low voltage electrostatic field (2 000 V/m, 2 500 V/m) on microbial changes of Cynoglossus semilaevis during storage. High-throughput sequencing techniques were used to determine and analyze samples of C. semilaevis from 0, 15, and 30 d storage. The results showed that the effective sequences of the samples ranged from 76 735~103 583 bp, with an average length of 428.39~429.17 bp and OTU numbers between 45~218. Microbial diversity and relative abundance decreased during storage for all groups of samples. At the phylum level, four microbial phyla were identified in each sample group, namely Proteobacteria, Firmicutes, Bacteroidota, and Others. 11 microbial genera were identified at the genus level. These were Acinetobacter, Psychrobacter, Shewanella, Brochothrix, Vagococcus, Photobacterium, Myroides, Carnobacterium, Pseudomonas, Achromobacter, and Others. At 30 d of storage, the dominant spoilage organisms in the CK group were Brochothrix, while the relative abundance of Brochothrix in samples from the LVEF-1 (2 000 V/m) and LVEF-2 (2 500 V/m) groups increased significantly but was lower than that of CK. The relative abundance of the CK group increased significantly, but was lower than that of the CK group, and the relative abundance of Psychrobacter and Shewanella still accounted for a certain proportion. It is assumed that the LVEF treatment was able to suppress the increase in the relative abundance of the dominant spoilage genera in the C. semilaevis. The results provide a theoretical basis for the effects of LVEF on microbial diversity and abundance changes during storage of C. semilaevis. |
| Key words: Cynoglossus semilaevis low voltage electrostatic field (LVEF) high-throughput sequencing bacterial diversity |
