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引用本文:郭锋,王玉霞,董震宇,姚荣辉,王伟峰,叶莹莹,严小军,郭宝英.高温胁迫下厚壳贻贝(Mytilus coruscus)消化腺代谢组学研究.海洋与湖沼,2024,55(1):171-181.
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高温胁迫下厚壳贻贝(Mytilus coruscus)消化腺代谢组学研究
郭锋1, 王玉霞2, 董震宇2, 姚荣辉2, 王伟峰2, 叶莹莹1, 严小军1,2, 郭宝英1,2
1.国家海洋设施养殖工程技术研究中心 浙江舟山 316022;2.浙江海洋大学海洋科学与技术学院 浙江舟山 316022
摘要:
在全球变暖的背景下, 海水温度不断升高、海洋暖化逐渐加剧, 高温严重影响着海洋生物的各种生命过程, 但对于海洋双壳贝类如何应对热应激的研究仍然不足。为此, 开展了以18 °C (CT)为对照组在26 °C (ST)和33 °C (HT)下对厚壳贻贝消化腺组织进行了急性热胁迫下的代谢组学分析,以便于研究其代谢反应。采用LC-MS/MS技术, 并结合生物信息分析手段对差异代谢物进行筛选, 并分析确定相关的代谢通路的变化, 共有2 532种代谢物在厚壳贻贝消化腺中被鉴定。KEGG富集分析用于探索差异代谢物的潜在代谢途径, 共有29条代谢通路被显著富集, 与对照组相比, ST组显著富集于牛磺酸和次牛磺酸代谢、神经活性配体-受体相互作用、鞘脂类代谢和视黄醇代谢等代谢通路; HT组显著富集于酪氨酸代谢、亚油酸代谢、丙氨酸新陈代谢、酪氨酸代谢、色氨酸代谢、苯丙氨酸代谢等代谢通路。研究结果显示, 厚壳贻贝消化腺主要通过调节色氨酸代谢、酪氨酸代谢、鞘脂代谢、苯丙氨酸代谢、氧化磷酸化, 脂肪酸、赖氨酸降解等信号通路应对热应激, 从而帮助维持身体内部环境的稳定状态。上述研究为多视角探究厚壳贻贝耐热机制与应对环境中温度变化的适应性进化提供理论基础。
关键词:  厚壳贻贝(Mytilus coruscus)  消化腺  高温胁迫  代谢组学
DOI:10.11693/hyhz20230900183
分类号:
基金项目:国家自然科学基金面上项目,42076119号;浙江省“三农九方”科技协作计划“揭榜挂帅”项目,2023SNJF065号。
附件
METABOLOMICS OF THE DIGESTIVE GLAND OF MYTILUS CORUSCUS UNDER HIGH-TEMPERATURE STRESS
GUO Feng1, WANG Yu-Xia2, DONG Zhen-Yu2, YAO Rong-Hui2, WANG Wei-Feng2, YE Ying-Ying1, YAN Xiao-Jun1,2, GUO Bao-Ying1,2
1.National Engineering Research Center for Marine Aquaculture, Zhoushan 316022, China;2.College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China
Abstract:
With the continuation of global warming, the problem of ocean warming has been gradually intensified, and high temperatures seriously affect various life processes of marine organisms. However, studies on how marine bivalve mussels cope with heat stress remain insufficient. A metabolomic analysis of digestive gland tissues of M. coruscus under acute heat stress was carried out at 26 °C and 33 °C against the control at 18 °C, to study the metabolic responses. LC-MS/MS technology was used, and differential metabolites were screened and analyzed for changes in relevant metabolic pathways. A total of 2 532 metabolites were identified in the digestive gland. KEGG enrichment analysis was used to explore potential metabolic pathways of differential metabolites, and 29 metabolic pathways were significantly enriched. Compared to the control group, the 26 °C group showed significant enrichment in taurine and hypotaurine metabolism, neuroactive ligand-receptor interaction, sphingolipid metabolism, and retinol metabolism, while the 33 °C group showed significant enrichment in the metabolisms of tyrosine, linoleic acid, alanine, tryptophan, phenylalanine, and phenylalanine. Results indicate that the digestive gland of M. coruscus responds to heat stress by mainly regulating tryptophan metabolism, tyrosine metabolism, sphingolipid metabolism, phenylalanine metabolism, oxidative phosphorylation, and degradation pathways of fatty acids and lysine to help maintain internal environmental stability. This research provided a theoretical basis for exploring the adaptative evolution of M. coruscus against temperature changes in the environment from multiple perspectives.
Key words:  Mytilus coruscus  digestive gland  high-temperature stress  metabolomics
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