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引用本文:王飞扬,陈素阳,解家松,姜建湖,郭建林,张海琪.美人鱼发光杆菌(Photobacterium damselae)感染对银鲳(Pampus argenteus)肠道微生物的影响.海洋与湖沼,2024,55(1):213-222.
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美人鱼发光杆菌(Photobacterium damselae)感染对银鲳(Pampus argenteus)肠道微生物的影响
王飞扬1,2, 陈素阳2, 解家松2, 姜建湖1, 郭建林1, 张海琪1
1.浙江省淡水水产研究所 浙江湖州 313001;2.宁波大学海洋学院 浙江宁波 315211
摘要:
银鲳(Pampus argenteus)是我国沿海重要的经济鱼类和水产养殖重要品种, 近年来因过度捕捞其野生种群数量日渐减少。美人鱼发光杆菌(Photobacterium damselae, PDD)是多种水产动物发病的主要病原菌, 也是银鲳养殖中的一种常见病原微生物, 会引起造成银鲳大规模死亡的出血病,但目前尚不清楚美人鱼发光杆菌感染后对银鲳肠道微生物的影响。通过注射使银鲳感染PDD, 在24 h和72 h分别采集健康及患病银鲳的中肠和后肠进行16S rRNA基因扩增子测序, 并结合Illumina Nova测序平台进行测序和数据分析, 研究PDD感染后不同时间银鲳体内微生物群落组成结构的动态变化。结果表明, PDD感染后在银鲳肠道内大量增殖并占据优势, 引起银鲳体内肠道微生物的动态变化。PDD的侵入及在银鲳肠道中的增殖并占据优势, 导致银鲳肠道内微生物群落组成结构紊乱。PDD的过度生长与肠道微生物群落构成失调密切相关, 显示了肠道微生物群落结构平衡的重要性。研究结果有助于深入探究水产动物肠道微生物的多样性、功能及相互关系, 为银鲳养殖业的健康管理提供科学依据。
关键词:  银鲳(Pampus argenteus)  肠道微生物  美人鱼发光杆菌(Photobacterium damselae)  疾病  群落结构平衡
DOI:10.11693/hyhz20230700136
分类号:Q789;S917.1
基金项目:国家自然科学基金项目,42106105号;浙江省“三农九方”科技协作计划项目,2023SNJF070号;宁波市公益性科技计划项目,2021S061号;宁波市甬江引才工程创新团队项目,2021B-029-C号。
附件
EFFECTS OF PHOTOBACTERIUM DAMSELAE INFECTION ON GUT MICROBES IN SILVER POMFRET PAMPUS ARGENTEUS
WANG Fei-Yang1,2, CHEN Su-Yang2, XIE Jia-Song2, JIANG Jian-Hu1, GUO Jian-Lin1, ZHANG Hai-Qi1
1.Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China;2.School of Marine Sciences, Ningbo University, Ningbo 315211, China
Abstract:
The intestinal microbiota of fish plays a vital role in the host. Photobacterium damselae subsp. damselae (PDD) is a common pathogenic microbe in cultured silver pomfret (Pampus argenteus), often cause massive mortality. However, the impact of PDD infection on the intestinal microbiota of P. argenteus during the development of PDD disease is unclear. In this study, P. argenteus was infected with PDD by intramuscular injection, and the midgut and hindgut of healthy and diseased P. argenteus were collected at 24 h and 72 h for 16S rRNA gene amplicon sequencing. The results showed that PDD proliferated and dominated in the intestinal tract of P. argenteus after infection, reducing the relative abundance of other microbial groups, disrupting the normal balance of the intestinal microbiota, and causing a disorder in the composition of the microbial community in the intestinal tract of P. argenteus.
Key words:  silver pomfret Pampus argenteus  intestinal microbiota  Photobacterium damselae  disease  balance of community structure
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