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引用本文:姜海峰,刘小林,常亚青,冷晓飞,李 丹,王高学.皱纹盘鲍(Haliotis discus hannai)肠道潜在益生菌的筛选及对幼鲍生长的影响.海洋与湖沼,2013,44(2):482-487.
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皱纹盘鲍(Haliotis discus hannai)肠道潜在益生菌的筛选及对幼鲍生长的影响
姜海峰1, 刘小林1, 常亚青2, 冷晓飞3, 李 丹3, 王高学1
1.西北农林科技大学动物科技学院;2.大连海洋大学 农业部海洋水产增养殖学重点实验室;3.大连太平洋海珍品有限公司
摘要:
采用体外实验筛选益生菌结合16S rDNA序列分析法, 从124株不具有溶血作用的皱纹盘鲍(Haliotis discus hannai)肠道细菌中筛选得到5株潜在益生菌并进行了分子鉴定, 进一步对5株潜在益生菌进行了安全性实验及体内饲喂实验。结果表明, 2 株具有拮抗哈维弧菌和灿烂弧菌能力的潜在益生菌分别被鉴定为Shewanella sp. (WA64)和Shewanella sp. (WA65), 一株产海藻酸酶和淀粉酶的潜在益生菌被鉴定为Vibrio sp. (WA51), 产蛋白酶潜在益生菌被鉴定为 Bacillus sp. (FA12), 产琼脂酶潜在益生菌被鉴定为Tamlana sp. (FA86); 安全性实验表明5株潜在益生菌在107cfu/ml下对皱纹盘鲍没有明显的毒害作用; 通过体内饲喂实验发现, 潜在益生菌WA6 4、WA6 5的复合作用能够显著提高幼鲍增重率和存活率(P<0.05), 并在生产条件下能够明显降低幼鲍的死亡量。经抗生素敏感性实验, WA6 4菌株对15种抗生素均敏感或中度敏感, WA65 菌株仅对庆大霉素和链霉素2种抗生素产生耐药。
关键词:  幼鲍  益生菌  筛选  生长
DOI:10.11693/hyhz201302034034
分类号:
基金项目:国家“863”计划项目, 2006AA10A411 号
附件
SELECTION OF POTENTIAL PROBIOTICS FROM ABALONE INTESTIONAL AND THEIR EFFECTS ON GROWTH OF JUVENILE SMALL ABALONE HALIOTIS DISCUS HANNAI
JIANG Hai-Feng1, LIU Xiao-Lin1, CHANG Ya-Qing2, LENG Xiao-Fei3, LI Dan3, WANG Gao-Xue1
1.College of Animal Science and Technology, Northwest Agriculture and Forestry University;2.Key Laboratory of Mariculture of Ministry of Agriculture, Dalian Ocean University;3.Dalian Pacific Fisheries Co., Ltd.
Abstract:
Five strains of potential probiotics isolated from 124 non-hemolysis strains of the GI of abalone in vitro were identified by 16S rDNA sequence analysis. In addition, pathogenicity and feeding experiment were conducted. Results show that Strain WA64 and WA65 which exhibit inhibitory effects against Vibrio harveyi,Vibrio splendidus are identified as Shewanella; WA51 produced amylase and alginase is identified as Vibrio; FA123 produced protease is identified as Bacillus; FA86 produced agarase is identified as Tamlana. Potential probiotics are safe to abalone under 107cfu/ml. Feeding experiment showed the weight gain ratio and survival rate were significantly increased in treatment supplemented with WA64 and WA65 (P<0.05). Furthermore, the effects of WA64 and WA65 in pilot scale trials show the mortality of juvenile small abalone decreases obviously. Antibiotic sensitivity assays revealed that strain WA64 was sensitive or medium sensitive to the 15 antibiotics tested, and WA65 was resistant to Gentamicin and Streptomycin.
Key words:  juvenile abalone Haliotis discus hannai  probiotics  screening  growth
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