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| 湛江湾沉积物中反硝化和厌氧氨氧化细菌的丰度、多样性及分布特征* |
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毛铁墙1, 董宏坡1,2, 陈法锦1, 侯庆华1, 朱庆梅1, 阳雯娜1, 张家伟1, 纪梓晗1, 凌炜琪1
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1.广东海洋大学海洋与气象学院;2.华东师范大学河口海岸国家重点实验室
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| 摘要: |
| 采用实时荧光定量PCR、高通量测序等方法对湛江湾沉积物中四个月份的反硝化细菌与厌氧氨氧化细菌的多样性和丰度进行了分析。结果表明：湛江湾沉积物中反硝化细菌和厌氧氨氧化细菌丰度在四个月份的变化和空间分布趋势为：nirS型反硝化细菌在二月份最高，四月份最低，且其平均丰度有从湛江湾湾内向湾口附近呈现先升高再降低的趋势；nirK型反硝化细菌丰度在九月份最高，十一月份最低；nosZ型反硝化细菌在四月份最高，其余月份变化不大；厌氧氨氧化细菌丰度在九月份最高，二月份最低；通过相关性分析结果表明，亚硝酸盐、铵盐等共同调控着湛江湾沉积物中反硝化和厌氧氨氧化细菌丰度变化。系统发育分析表明：湛江湾中存在着一些广泛分布的反硝化细菌，但也生活着一些新奇的nirK型和nosZ型反硝化细菌。对于厌氧氨氧化细菌而言，其主要属于浮霉菌门及Candidatus Scalindua属，具有较高的耐盐性，另外湛江湾海区的厌氧氨氧化细菌也生活着一类在其它地方没有的新分支。CCA分析结果表明：硝酸盐显著影响湛江湾反硝化细菌和厌氧氨氧化细菌的群落结构。湛江湾沉积物中反硝化细菌和厌氧氨氧化细菌存在特殊的竞争与共存的关系，且由亚硝酸盐、硝酸盐、pH等多种环境因子共同驱动。 |
| 关键词: 反硝化和厌氧氨氧化细菌 实时荧光定量PCR 高通量测序 多样性 丰度 湛江湾 |
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| 基金项目:国家自然科学基金，41576123,41971125； 广东省自然科学基金，2018A030313164 |
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| Abundance, Diversity and Distribution Characteristics of Denitrifier and Anammox Bacteria in Sediments of Zhanjiang Bay |
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Mao Tieqiang1, Dong Hongpo1,2, Chen Fajin1, Hou Qinghua1, Zhu Qingmei1, Yang Wenna1, Zhang Jiawei1, Ji Zihan1, Ling Weiqi1
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1.College of Ocean and Meteorology, Guangdong Ocean University;2.State Key Laboratory of Estuarine and Coastal Research, East China Normal University
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| Abstract: |
| The diversity and abundance of denitrifying bacteria and anaerobic ammonium oxidizing bacteria in the sediments of Zhanjiang Bay were analyzed by real-time fluorescent quantitative PCR and high-throughput sequencing. The results showed that the abundance of denitrifying bacteria and anammox bacteria in the sediments of Zhanjiang Bay changed significantly with season and spatial. The nirS-type denitrifying bacteria were the highest in February and the lowest in April, and its average abundance tends to increase first and then decrease from inside to outside of Zhanjiang Bay; the abundance of nirK-type denitrifying bacteria were the highest in September and the lowest in November; nosZ-type denitrifying bacteria were the highest in April, and did not change in other months; the abundance of anammox bacteria were the highest in September and the lowest in February. By correlation analysis showed that nitrite and ammonium jointly regulated the changes of denitrifying and anammox bacteria abundances in sediments of Zhanjiang Bay. Phylogenetic analysis showed that there are some widely distributed denitrifying bacteria in Zhanjiang Bay, but certain novel nirK and nosZ type denitrifying bacteria were also found. For anaerobic ammonium oxidizing bacteria, it mainly belongs to the Planctomycetes and Candidatus Scalindua, which have high salt tolerance. In addition, a new branch of the anaerobic ammonium oxidizing bacteria inhabited in Zhanjiang Bay which are not found elsewhere. CCA analysis showed that nitrate significantly affected the community structure of denitrifying bacteria and anaerobic ammonium oxidizing bacteria in Zhanjiang Bay. There are competition between denitrifying bacteria and anaerobic ammonium oxidizing bacteria, and they are driven by various environmental factors such as nitrite, nitrate and pH. |
| Key words: Denitrifier and anammox bacteria Real-time quantitative PCR High-throughput sequencing diversity abundance Zhanjiang bay |