| 摘要: |
| 为研究池塘内循环流水养殖(In-pond Raceway, IPR)模式下太湖鲂鲌(翘嘴鲌(♀)×团头鲂(♂))肠道微生物群落结构的变化,以传统池塘养殖作为对照组,采用16S rRNA高通量测序方法分析了IPR养殖模式下太湖鲂鲌肠道的菌群结构及环境水体微生物多样性的变化。试验结果表明,IPR模式下太湖鲂鲌肠道微生物群落发生了明显的变化,在门分类水平,梭杆菌门(Fusobacteria)成为绝对优势菌,所占丰度为92.47%；对照组的优势菌由梭杆菌门(Fusobacteria)、变形菌门(Proteobacteria)和厚壁菌门(Firmicutes)组成,三者所占的丰度分别为34.45%、33.30%和21.30%。在养殖水环境中,两种养殖模式的微生物群落数均大于肠道样本,且二者优势菌不同；IPR水环境的优势菌为蓝细菌(Cyanobacteria,36.53%),其次为放线菌门(Actinobacteria,24.67%)；对照组水体中的优势菌为变形菌门(Proteobacteria)和放线菌门(Actinobacteria),分别占细菌总数的38.99%和28.15%。多样性指数结果表明,水环境中的微生物群落Shannon多样性指数、Chao1指数高于肠道样本,IPR养殖对象肠道微生物群落多样性最低。本研究结果揭示,池塘内循环流水高密度养殖模式下,太湖鲂鲌的肠道微生物结构发生了一定的改变,微生物群落多样性降低,应注意该模式下养殖对象的肠道健康,加强养殖管理。 |
| 关键词: 太湖鲂鲌(翘嘴鲌(♀)×团头鲂(♂)) 肠道微生物 池塘内循环流水养殖(IPR) 菌群结构 高通量测序 微生物群落多样性 |
| DOI： |
| 分类号: |
| 基金项目:浙江省重点研发计划项目, 2018C02033号; 浙江省自然科学基金项目, 2019YZ12号。 |
附件 |
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| STUDAY ON THE CHANGES OF INTESTINAL MICROBIOTA OF A NEW HYBRID STRAIN OF (FEMALE C. ALBURUUS) × (MALE M. AMBLYCEPHALA) REARED IN IPR AQUACULTURE SYSTEM |
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LI Qian,GUO Jian-Lin,WANG Yu-Chen,JIANG Jian-Hu,SUN Li-Hui,MI Guo-Qiang,CHEN Jian-Ming
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Zhejiang Institute of Freshwater Fisheries,Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture
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| Abstract: |
| In order to study the changes of intestinal microbiota in a new hybrid strain of (female Culter alburuus Bssilewsky × male Megalobrama amblycephala Yih) reared in the in-pond race (IPR) aquaculture system, 16S rRNA high-throughput sequencing method was used to analyze the microflora structure and diversity of intestinal tract and water samples while the common pond was used as a control group. The results showed that the microbial structure of intestinal tract had been changed obviously in the IPR group and it was strongly dominated by Fusobacteria on the phylum level, which occupied for 92.47% of the total species. However, there were three dominant phyla in the control group which included Fusobacteria, Proteobacteria and Firmicutes, the abundance percents of the three dominant phyla were 34.45%, 33.30% and 21.30%, respectively.The numbers of microflora in water samples were higher than that of intestinal samples, and their dominant phylum was different. Cyanobacteria was the most abundant phylum in the water of IPR, the relative abundance was 36.53%. Actinobacteria was the second dominant phylum which occupied 24.67%. In contrast, Actinobacteria and Proteobacteria were the most abundant phyla in the control group and their relative abundances were 38.99% and 28.15%, respectively. The diversity index indicated that the Shannon index and Chao1 index were higher in water samples than in that of intestinal tract, and the value of the diversity index in the IPR intestinal sample was the lowest in all of the samples. The results revealed that the structure of intestinal microflora in the IPR had changed to a certain extent and the diversity of bacterial communities decreased obviously. Attention must be paid to the intestinal health and culturing management of the IPR. |
| Key words: female Culter alburuus Bssilewsky × male Megalobrama amblycephala Yih intestinal microbiota in-pond raceway (IPR) aquaculture bacterial communities structure high-throughput sequencing diversity of microflora communities |