| 摘要: |
| 为探究企鹅珍珠贝贝壳内表面珍珠质层不同区域颜色差异形成的原因,我们选取9个生物矿化相关基因,利用实时荧光定量PCR扩增技术对其在外套膜不同部位的表达水平进行测定,并利用扫描电子显微镜对贝壳珍珠质层不同区域的内表面及纵断面的微观结构进行观察。结果表明：有8个矿化基因在企鹅珍珠贝外套膜远端膜区和中央膜区的表达量具有显著差异(P<0.05)；贝壳珍珠质层内表面颜色不同的两个区域(生长区和中心区)表面的超微结构和纵断面文石板片厚度均存在差异。我们推测,矿化基因可能是通过在外套膜不同区域的差异表达,影响企鹅珍珠贝珍珠质的形成,进而影响珍珠质层的颜色。而文石板片厚度很可能与贝壳珍珠质层颜色无关,而是与贝壳珍珠质层分泌的早晚有关。我们的研究为探究企鹅珍珠贝珍珠质层内表面颜色显著不同区域的形成机制提供理论基础。 |
| 关键词: 企鹅珍珠贝 珍珠质层 矿化基因 扫描电子显微镜 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
附件 |
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| Study on the effect of gene expressions and shell ultrastructure on the color of nacreous layer in Pteria penguin |
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Zhang Jiayi, Yang Lei, Chen Yi, Wang Aimin, Zhan Xin
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College of Marine Science, Hainan University
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| Abstract: |
| In order to investigate the mechanism of different colors in shell nacreous areas in Pteria penguin, real-time fluorescent quantitative PCR amplification technology were used to determine nine biomineralization genes expression levels in different parts of the mantle. And the microstructure of shell nacreous layer with different colors were observed by the scanning electron microscope. The thickness of aragonite sheet in different shell areas were measured by ImageJ. The results showed that there were significant differences in the expression levels of 8 biomineralization genes among the two areas of the mantle in Pteria penguin (P<0.05); and the growth area and central area on the inner surface of the nacre have significantly different microstructure (P<0.05). The biomineralization genes may influence the structure of nacreous layer and thickness of aragonite sheet, while nacreous color may impaired by the surface structure of shell. Our research provides valuable information for illustrating the mechanism of the color formation of shell nacreous layer in Pteria penguin. |
| Key words: Pteria penguin nacreous layer biomineralization genes scanning electron microscope |