首页 | 期刊简介 | 编委会 | 投稿指南 | 常用下载 | 联系我们 | 期刊订阅 | In English
引用本文:
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 47次   下载 0  
分享到: 微信 更多
α-酮戊二酸及其钠盐对雨生红球藻生长和虾青素积累的促进作用
张豪杰1, 李旺宁1, 梁梦静1, 李亚男1, 张春辉1, 季春丽1, 李润植1, 崔玉琳2, 秦松2, 崔红利1
1.山西农业大学;2.中国科学院烟台海岸带研究所
摘要:
雨生红球藻是已知虾青素(astaxanthin, AST)含量最高的生物,是理想的细胞制备工厂。尽管研究表明添加α-酮戊二酸(OG, α-oxoglutarate)能有效促进混养条件下雨生红球藻AST积累,但在自养条件下是否也有类似效果不清楚。本研究以自养雨生红球藻为对象,在正常(CK)、高光(HL)、氮缺乏(ND)和高光-氮缺乏双重逆境(HL-ND)下从细胞生长和AST积累两方面评估OG及其钠盐(OG-2Na)的促进作用。结果显示:添加OG或OG-2Na显著缓解HL、ND和HL-ND胁迫对细胞生长的抑制作用,培养至6 d的生物量分别为0.58、0.53和0.38 g/L,约是未添加组的2.0倍。添加OG或OG-2Na显著促进AST积累。培养至6 d,在HL、ND和HL-ND下,AST含量分别达到13.62、19.51和28.29 mg/g,是未添加组的2.39、1.16和1.35倍。同时在胁迫条件下,添加OG或OG-2Na有效提高单位细胞中AST含量。针对OG和OG-2Na添加,最大单位细胞AST含量分别出现在HL和HL-ND条件下,达到53.72 和60.58 pg/cell,是对照的3.09和2.83倍。在CK下,添加10.0 mg/L的OG或OG-2Na,其AST含量显著高于单独的胁迫条件。上述结果证实OG或OG-2Na不但可减少HL、ND和HL-ND胁迫对红球藻细胞生长的抑制、显著提高其AST含量,是一种在提高AST产量的有效途径。
关键词:  雨生红球藻  α-酮戊二酸  生长  虾青素积累  促进作用
DOI:
分类号:
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
Promoting effects of α-ketoglutarate and its sodium salt on growth and astaxanthin accumulation in Haematococcus pluvialis
Zhang Hao Jie1, Li Wang Ning1, Liang Meng Jing1, Li Ya Nan1, Zhang Chun Hui1, Ji Chun Li1, Li Run Zhi1, Cui Yu Lin2, Qin Song2, Cui Hong Li1
1.Shanxi Agricultural University;2.Key Laboratory of Coastal Biology and Biological Resources Utilization
Abstract:
Haematococcus pluvialis has been considered an ideal cell factory for AST production due to the highest AST content. Although it has been reported that adding α-ketoglutaric acid (OG) can effectively promote AST accumulation in algal cells under mixotrophic condition, its effect on autotrophic cells is unclear. In this study, autotrophic H. pluvialis cells were cultured under normal conditions (CK, Control), high light (HL), nitrogen deficiency (ND) and high light and nitrogen deficiency (HL-ND). The effects of different concentrations of OG and its disodium salt (OG-2Na) on growth and AST content were evaluated. The results showed that compare with CK, the addition of OG or OG-2Na could significantly enhance the growth of algal cells under HL, ND and HL-ND stresses. After 6 days of culture, the maximum biomass content under HL, ND and HL-ND stresses was 0.58 g/L, 0.53 g/L and 0.38 g/L, respectively, which were approximate 2.0 times that of the group without addition under the same conditions. Interestingly, the accumulation of AST was significantly promoted by adding OG or OG-2Na under stress conditions. After 6 days of culture, the maximum AST contents under HL, ND and HL-ND stresses were 13.62 mg/g, 19.51 mg/g and 28.29 mg/g, respectively, which were 2.39, 1.16 and 1.35 times of those in the non-supplemented group under the same conditions. Under stress conditions, the AST content per cell was significantly increased by adding OG or OG-2Na. For OG and OG-2Na supplementation, the highest AST content per cell reached 53.72 pg/cell and 60.58 pg/cell under HL and HL-ND conditions, respectively, which were 3.09 and 2.83 times of those without supplementation under the same condition. It is worth noting that AST content under CK condition with 10.0 mg/L OG or OG-2Na addition was significantly higher than that under the same stresses without supplement. In conclusion, the addition of OG or OG-2Na not only significantly relieved the inhibition of HL, ND and HL-ND stress conditions on the growth of algal cells, but also significantly increased AST content, which provided a new way to increase AST production under normal conditions in H. pluvialis.
Key words:  Haematococcus pluvialis  α-Ketoglutarate  Growth  Astaxanthin accumulation  Promoting effect
版权所有 海洋与湖沼 Oceanologia et Limnlolgia Sinica Copyright©2008 All Rights Reserved
主管单位:中国科协技术协会 主办单位:中国海洋湖沼学会
地址:青岛市福山路32号  邮编:266071  电话:0532-82898753  E-mail:ols@qdio.ac.cn
技术支持:北京勤云科技发展有限公司