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溶藻弧菌体外刺激后泥蚶血细胞早期转录组研究
钟爱华, 毕澜方, 徐浩磊
浙江海洋大学水产学院 舟山 316022
摘要:
泥蚶是我国传统养殖贝类,细菌性疾病导致的死亡已成为制约泥蚶养殖业发展的重要因素,血细胞是泥蚶免疫防御的执行者,血细胞能直接吞噬异物,能通过包囊作用将异物包裹,血细胞还在炎症反应和伤口修复中发挥作用。本文采用RNA-seq测序技术研究了溶藻弧菌体外刺激后泥蚶血细胞3h和6h时转录组动态变化,与未受刺激血细胞相比,3h时1 790个基因表达发生显著改变,包括1 319个上调基因和471个下调基因;6h时3 183个基因表达发生显著改变,包括2 629个上调基因和554个下调基因。KEGG通路富集分析发现溶藻弧菌刺激后免疫相关多个信号通路或生物学过程发生显著改变,如吞噬体、细胞凋亡、蛋白酶体、内质网加工、局部粘附等。部分免疫相关基因表达丰度3h时变化不显著而6h时发生显著改变,部分免疫相关基因3h和6h时表达丰度均显著改变。该结果为泥蚶血细胞早期免疫反应提供了新的见解,为泥蚶抗病机理研究提供了的理论基础。
关键词:  泥蚶  溶藻弧菌  血细胞  转录组
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The transcriptomic analysis of the in vitro hemocytes of blood clam (Tegillarca granosa) challenged with Vibrio alginolyticus
zhongaihua, Bilanfang, Xvhaolei
zhejiang ocean university
Abstract:
Blood clam (Tegillarca granosa) is a traditional cultured shellfish in China. The death caused by bacterial diseases has become an important factor restricting the development of clam breeding industry. The hemocytes are the executor of immune defense. The hemocytes can directly swallow foreign bodies and wrap them up through encapsulation. The hemocytes also play a role in inflammatory reaction and wound repair. In this paper, RNA-seq sequencing was used to study the dynamic changes of transcriptome of in vitro hemocytes stimulated by Vibrio alginolyticus at 3h and 6h. Compared with unstimulated hemocytes, the expression of 1790 genes were significantly changed at 3h, including 1319 up-regulated genes and 471 down-regulated genes. At 6h, the expression of 3183 genes changed significantly, including 2629 up-regulated genes and 554 down regulated genes. KEGG pathway enrichment analysis found that many immune-related signal pathways or biological processes changed significantly after V. alginolyticus stimulation, such as phagosome, apoptosis, proteasome, endoplasmic reticulum processing, local adhesion and other pathways. The expression abundance of some immune-related genes did not change significantly at 3h, but changed significantly at 6h, and the expression abundance of some immune-related genes changed significantly at 3h and 6h. The results provide a new insight into the early immune response of the hemocytes of blood clam and a theoretical basis for the study of disease resistance mechanism of blood clam.
Key words:  Tegillarca granosa  Vibrio alginolyticus  hemocytes  transcriptome
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