首页 | 期刊简介 | 编委会 | 投稿指南 | 常用下载 | 联系我们 | 期刊订阅 | In English
引用本文:
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 78次   下载 0  
分享到: 微信 更多
不同程度强光胁迫对海带幼苗光合生理影响研究*
牛建峰,冯泽中,孙振杰,王伟伟,张晓雯,梁广津,王立军,李晓捷,王广策
1.中国科学院海洋研究所;2.青岛农业大学;3.山东东方海洋科技股份有限公司,山东省海藻遗传育种与栽培技术重点实验室;4.中国水产科学研究院黄海水产研究所
摘要:
海带(Saccharina japonica)是我国藻类生产的主要品种之一,其栽培产量和规模均居世界首位。2021-2022年产季,山东荣成海带主产区先后爆发了大规模的病烂灾害,给当地水产经济造成了巨大损失。引起病烂的原因,可能来自多方面,本文就光照强度及海水营养水平对海带生理的影响进行了测定,以期为烂菜现象的病因分析提供一定的线索。基于幼体海带最小饱和光强、烟台高新区牟平自然海区海水氮磷浓度及不同海水深度下的光照强度测定结果,研究了梯度强光在不同海水营养盐水平下对海带生理的影响。结果显示幼体海带在700~900 μmol m-2 s-1强光胁迫3天后,Fv/Fm 可恢复至对照水平,光合色素合成活跃,营养盐含量较高的海水更有助于PSII的修复,但1300~1500 μmol m-2 s-1的强光辐射导致PSII不能恢复,岩藻黄素、叶绿素a及β-胡萝卜素含量显著降低。整个胁迫实验中,藻体总抗氧化能力(T-AOC)及其它抗氧化酶比活力大体上在强光胁迫的起始至第3天上调,而在胁迫第5天时又出现下降趋势,且在营养盐含量较高的处理组中,各样本活性均高于天然海水处理组样本。抗氧化酶基因的表达也呈现类似的变化趋势。在低于900 μmol m-2 s-1的光照条件下,抗氧化酶活性可以得到较好的维持,而海水中相对较为丰富的营养盐则有利于藻体抗氧化能力的维持。
关键词:  强光胁迫  活性氧清除  光系统修复  海带  抗氧化酶  岩藻黄素  β-胡萝卜素
DOI:
分类号:Q539;Q51
基金项目:国家重点研发计划“蓝色粮仓科技创新”重点专项 (2018YFD0901500)
Effects of different degrees of strong light stress on Photosynthetic Physiology of Saccharina japonica seedlings
NIU Jian-feng1, FENG Zezhong2, SUN Zhenjie2, WANG Weiwei3, ZHANG Xiaowen4, LIANG Guangjin3, WANG Lijun1, LI Xiaojie3, WANG Guangce5
1.Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences;2.Qingdao Agricultural University;3.Provincial Key Laboratory of Algae Genetic Breeding and Cultivation Techniques of Shandong, Shandong Oriental Ocean Sci-tech Co. Ltd;4.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;5.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Abstract:
Saccharina japonica is one of the main species of algae production in China, and its cultivation scale and yield rank first in the world. In the annual production season of 2021-2022, a large-scale disease disaster broke out in the area of Shandong Rongcheng, causing huge losses to the local aquatic economy. The causes of disease may come from many aspects. Here, the effects of light intensity and nutritional level of seawater on kelp physiology were determined. This will provide some clues for the analysis of the disaster. Based on the results of the minimum saturated light intensity of S. japonica, the concentrations of nitrogen and phosphorus in natural seawater and the light intensity at different depths, the effects of strong light on the physiology of S. japonica at different seawater nutrient levels were studied. Results showed that the PSII of the seedlings of S. japonica could be maintained well under 700~900 μmol m-2 s-1 for a long time and the relatively high nutrient level of seawater contributed to the repairment of PSII under high light stress conditions. However, under the light condition of 1300~1500 μmol m-2 s-1, the recovery of PSII was damaged and the contents of fucoxanthin, chlorophyll a and β-carotene decreased significantly. In the whole stress experiment, the total antioxidant capacity (T-AOC) and specific activities of antioxidant enzymes of algae were generally up-regulated from the beginning of treatment to the third day, but decreased on the fifth day of the experiment. Moreover, the enzyme activities of the algae incubated with nutrient seawater were higher than those incubated with the natural seawater. The expression of antioxidant enzyme genes also showed a similar trend. In summary, the antioxidant enzyme system was active for several days below 900 μmol m-2 s-1 and the addition of exogenous nutrients could extend the activity maintenance period.
Key words:  High light stress  Scavenging of reactive oxygen species  Repairment of photosystem II  Saccharina japonica  Antioxidas  Fucoxanthin  β-carotene
版权所有 海洋与湖沼 Oceanologia et Limnlolgia Sinica Copyright©2008 All Rights Reserved
主管单位:中国科协技术协会 主办单位:中国海洋湖沼学会
地址:青岛市福山路32号  邮编:266071  电话:0532-82898753  E-mail:ols@qdio.ac.cn
技术支持:北京勤云科技发展有限公司