| 摘要: |
| 缢蛏养殖经由肥水塘投放大量含氮化合物，了解沉积物氮转化过程对调控和管理河口养殖塘氮的收支具有重要意义。以福建九龙江口肥水-缢蛏养殖塘为研究对象，运用同位素示踪技术和高通量测序技术探究缢蛏塘和肥水塘沉积物厌氧氨氧化脱氮速率及微生物群落结构，并分析其环境调控机制。结果表明：肥水塘沉积物厌氧氨氧化速率为1.76 ± 0.11 nmol/(g·h)，相对贡献为9.16% ± 0.40%，显著高于缢蛏塘（P < 0.05)。缢蛏塘和肥水塘厌氧氨氧化微生物主要由Ca.Scalindua、Ca.Kuenenia、Ca.Brocadia和Ca.Anammoximicrobium属组成。其中，Ca.Kuenenia属为养殖塘的优势菌属。沉积物厌氧氨氧化微生物多样性和丰富度呈显著的空间分布，肥水塘中生物多样性更高（P < 0.05）。厌氧氨氧化微生物活性和群落结构差异主要受养殖土地利用方式、溶解氧（DO）、NH4+和NO3-含量等环境因子的调控，一定程度上揭示了养殖环境中厌氧氨氧化过程的重要性，填补了河口养殖湿地氮回归大气的认知。 |
| 关键词: 厌氧氨氧化 养殖塘 速率 群落结构 影响因素 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金(41401297)；自然资源部第三海洋研究所开放基金(GCMAC2008) |
附件 |
|
| Study on the activity of anaerobic ammonia oxidizing bacteria from special Fertilizer-Sinonovacula constricta aquaculture ponds in Jiulong River Estuary |
|
Cai Lu1, Weng Bosen1, Zhan Liyang2, Zhang Jiexia2, Hu Gongren1, Yu Ruilian1, Yan Yu1
|
|
1.School of Chemical Engineering, Huaqiao University;2.Key Laboratory of Global Change and Marine-Atmospheric Chemistry, Third Institute of Oceanography
|
| Abstract: |
| A large amount of nitrogen compounds were put into Sinonovacula constricta ponds through fertilizer ponds. Understanding the nitrogen (N) removal process of sediments plays an important role in regulating and managing the balance of N in estuarine aquaculture ponds. Here this study reported the rates, microbial community structure of anaerobic ammonium oxidation (anammox) and its response to environmental factor using 15N isotope tracing technology and high-throughput sequencing methods in the Sinonovacula constricta- Fertilizer ponds along the coast of Fujian Province. Results showed that the potential rates of anammox in sediments of retirement ponds were 1.76 ± 0.11 nmol/(g·h), contribution rate was 9.16% ± 0.40%, which was significantly higher than the Sinonovacula constricta ponds. Anammox bacteira in the Sinonovacula constricta ponds and the fertilizer ponds were dominated by Ca.Scalindua, Ca.Kuenenia, Ca.Brocadia and Ca.Anammoximicrobium. The diversity and richness of anammox bacteria in the sediments of the fertilizer ponds were significantly higher than those of the Sinonovacula constricta ponds (P < 0.05). The stable aquaculture environment in the sediment of the fertilizer ponds created a powerful condition for the growth of anammox bacteria. Environmental factors including DO, NH4+, and NO3- content mainly regulated anammox microbial community structure. The community structure and distribution of anammox bacteria in the sediments of the Sinonovacula constricta ponds and the fertilizer ponds revealed the importance of the N removal process of anammox to a certain extent, and filled in the recognition of N return to the atmosphere in estuarine aquaculture wetland. |
| Key words: Anammox Aquaculture ponds Nitrogen removal rates Community structure Impact factor |