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| pH胁迫对菲律宾蛤仔(Ruditapes philippinarum)耗氧率、排氨率、免疫和抗氧化酶活以及组织形态的影响* |
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陈 强1,2, 林 毅1,2, 黎中宝1,2, 黄张帆1,2, 孔鲁闽1,2, 周思顺1,2, 龙中英1,2
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1.集美大学水产学院;2.福建省海洋渔业资源与生态环境重点实验室
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| 摘要: |
| 海洋酸化效应严重危害了海洋生物的生态健康,目前有关酸化对菲律宾蛤仔(Ruditapes philippinarum)生理生化影响的报道较少。为了探究菲律宾蛤仔对海洋酸化的反应,其养殖管理提供有效可靠的数据,利用HCl模拟海洋酸化的进程,研究pH值变化对菲律宾蛤仔排氨和耗氧率、鳃组织结构以及鳃和内脏团的免疫、抗氧化酶活的变化。结果表明：将菲律宾蛤仔置于酸化海水(pH 6.4、7.1和7.7)中,以自然海水(pH 8.1)为对照,蛤仔鳃组织结构损伤严重,鳃丝间距扩大,低酸化组纤毛粘合；鳃组织溶菌酶和碱性磷酸酶第42天酸化组含量显著高于对照组(P<0.05),而酸性性磷酸酶含量显著低于对照组(P<0.05),超氧化物歧化酶、总抗氧化能力和过氧化氢酶含量显著高于对照组(P<0.05),丙二醛和谷胱甘肽过氧化物酶含量显著低于对照组(P<0.05)；内脏团溶菌酶含量显著高于对照组(P<0.05),碱性和酸性磷酸酶与对照组无差异性,总抗氧化能力和超氧化物歧化酶含量显著高于对照组(P<0.05),过氧化氢酶和谷胱甘肽过氧化物酶与对照组无差异性,丙二醛低于对照组(P<0.05)。酸化致使菲律宾蛤仔排氨率和耗氧率降低,减缓其生理活动,降低鳃组织的免疫和抗氧化酶活性,打破蛤仔原有的代谢水平,会增加蛤仔患病的可能性。 |
| 关键词: pH 菲律宾蛤仔(Ruditapes philippinarum) 排氨耗氧率 免疫 抗氧化 |
| DOI：10.11693/hyhz20230100013 |
| 分类号: |
| 基金项目:福建省自然科学基金项目, 2020J01668号 |
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| EFFECTS OF pH STRESS ON OXYGEN CONSUMPTION RATE, AMMONIA EXCRETION RATE, IMMUNE AND ANTIOXIDANT ENZYME ACTIVITY AND TISSUE MORPHOLOGY OF CLAM RUDITAPES PHILIPPINARUM |
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Chen Qiang1,2, Lin Yi1,2, Li Zhongbao1,2, Huang Zhangfan1,2, Kong Lumin1,2, Zhou Sishun1,2, Long Zhongying1,2
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1. Fisheries College, Jimei University, Xiamen 361021, China;2. Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen 361021, China
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| Abstract: |
| With the rapid development of human industry, a large amount of carbon dioxide emissions have caused the acidification effect of the oceans, which not only leads to global warming, but also seriously endangers the ecological health of marine life. In this study, HCl was used to simulate the process of ocean acidification to study the effects of pH changes on ammonia excretion and oxygen consumption rate, gill tissue structure, and immune and antioxidant enzyme activity in the gill and visceral masses of Ruditapes philippinarum. When Ruditapes philippinarum were placed in acidified seawater (pH 6.4, 7.1 and 7.7) and natural seawater (pH 8.1) , the results showed severe damage to the gill tissue structure, enlarged gill filament spacing, and cilium bonding in the low acidification group. The contents of lysozyme and alkaline phosphatase in gill tissue of 42-day acidizing groups were significantly taller than those in the control group (P<0.05), less acidic phosphatase than control (P<0.05), there were differences between superoxide dismutase, total antioxidant capacity and catalase content compared with the control group (P<0.05), compared with the control group malondialdehyde and glutathione peroxidase levels were significantly inferior (P<0.05); the contents of visceral lysozyme were significantly outdistance the control group (P<0.05), in comparison with the control group alkaline phosphatase, acid phosphatase, catalase and glutathione peroxidase were not different, total antioxidant capacity and superoxide dismutase contents were significantly different from the control group (P<0.05), compared with the control group malondialdehyde was far from sufficient. (P<0.05). Acidification reduces the rate of ammonia excretion rate and oxygen consumption rate of Ruditapes philippinarum, slows down their physiological activity, reduces the immune and antioxidant enzyme activities of gill tissue, breaks the original metabolic level of Ruditapes philippinarum and increases the possibility of disease. |
| Key words: pH Ruditapes philippinarum ammonia oxygen consumption rate immunity antioxidant |
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