| 摘要: |
| 为揭示不同抗流能力的大黄鱼(Larimichthys crocea)肌肉基因表达水平的变化,筛选抗流相关基因,解析大黄鱼抗流性状形成的分子基础,收集福建宁德沙埕湾主养区1 000尾大黄鱼,在设计制作的抗流实验水槽中,以流速1.0 m/s为筛选条件,将抗流时间 > 30 min的大黄鱼归为抗流组(HM组),抗流时间 < 5 min的大黄鱼归为非抗流组(SM组),对其肌肉进行转录组分析,并统计抗流分组后48 h内大黄鱼的累计死亡率。转录组结果显示,HM组与 SM组文库共富集到1 806个差异表达基因(DEGs),其中1 090个上调,716个下调。GO 功能注释发现显著富集的条目主要集中在肌肉收缩相关功能,KEGG 富集分析发现上调的DEGs主要富集在心肌收缩、氧化磷酸化、黏着斑、ECM-受体相互作用、AGE-RAGE、MAPK、心肌细胞中的肾上腺素能等相关通路；下调的DEGs主要富集在真核生物核糖体的发生、蛋白酶体、内质网中的蛋白加工、RNA转运、泛素介导的蛋白水解信号通路等。此外,RT-qPCR结果表明,随机选取的DEGs与RNA-seq结果的表达趋势一致。48 h的累计死亡率结果显示,SM组大黄鱼的死亡率显著高于HM组(P<0.05)。由本研究得出,抗流能力强的大黄鱼可能通过改变细胞内能量代谢及肌肉收缩的方式来提高机体应对水流的能力,且在较强水流刺激后能够获得较高的成活率,这为后续培育适宜深远海养殖的大黄鱼抗流新品种提供了理论依据。 |
| 关键词: 大黄鱼 抗流性状 转录组 差异表达基因 |
| DOI：10.11693/hyhz20230200034 |
| 分类号: |
| 基金项目:中国水产科学研究院基本科研业务费项目, 2020XT1001号; 财政部和农业农村部国家现代农业产业技术体系, CARS-47号; 中国水产科学研究院东海产研究所基本科研业务费, 2021Z01号。 |
附件 |
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| TRANSCRIPTOME ANALYSIS OF MUSCLE TISSUE IN LARGE YELLOW CROAKER WITH DIFFERENT FLOW RESISTANCE |
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ZHANG Jing-Jing, WANG Ya-Bing, WANG Qian, HAN Duo-Cai, PENG Shi-Ming
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Key Laboratory of East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences
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| Abstract: |
| In order to reveal the varies of gene expression level with different flow-resistance ability, screening the flow-resistance related genes and analyzing the molecular basis of flow resistance in Larimichthys crocea, the experiment collected 1 000 large yellow croaker from the main culture area, Shacheng Bay, Ningde city, Fujian province. In the designed and fabricated flow resistance experimental flume, with the water flow velocity of 1.0 m/s, the large yellow croaker with flow resistance swimming time more than 30 min was classified as the flow-resistance group (HM group), whereas less than 5 min was classified as the non-flow-resistant group (SM group). Transcriptome analysis on its muscles and statistical cumulative mortality of large yellow croaker within 48h after grouped. A total of 1 806 differentially expressed gene (DEGs) were screened between HM and SM group libraries, including 1 090 up-regulated and 716 down-regulated genes. GO functional annotation found that significantly enriched entries mainly focused on muscle contraction-related functions. The up-regulated DEGs were significantly enriched in cardiac muscle, oxidative phosphorylation signal pathway, focal adhesion, ECM-receptor interaction, AGE-RAGE signaling pathway, MAPK signaling pathway, adrenergic signaling in cardiomyocytes. The down-regulated DEGs were mainly enriched in ribosome biogenesis in eukaryotes, proteasome, protein processing in endoplasmic reticulum, RNA transport, ubiquitin mediated proteolysis, etc. Furthermore, the randomly selected DGEs for RT-PCR and the results were consistent with the expression trend of the RNA-seq. The cumulative mortality results within 48 h showed that the SM group mortality rate was significantly higher than that in the HM group (P<0.05). Therefore, the stronger flow-resistance ability may improve the body"s ability to cope with flow by changing the way of intracellular energy metabolism and muscle contraction and enhance survival rate after strong current stimulation in L. crocea. These results will provide a theoretical basis for the subsequent cultivation of new varieties of large yellow croaker suitable for far-reaching aquaculture. |
| Key words: Larimichthys crocea flow-resistance trait transcriptome differential expression genes differentially expressed genes |