| 摘要: |
| 异帽藻属包含多种可致有毒有害赤潮的物种，其中最近在福建近海赤潮中分离的倪氏异帽藻(Hetercapasa niei)被证明有毒，然而对其超微形态结构，特别是对该属重要的分类保守特的体鳞，研究还不充分。本研究利用多种形态学方法和ITS片段分析了一株从厦门湾附近分离得到的藻株(SIO-H3)，结果证明该藻株属于倪氏异冒藻，其细胞呈双锥形，长14.3-18.2 μm，宽8.4-10.9 μm, 甲板方程式为Po, cp, 5', 3a, 7", 6c, 5s, 5', 2""。同时，发现了两个与之前描述不同的形态特征，该藻株细胞具有多个蛋白核(pyrenoids)和两种体鳞(body scale)结构：一种体鳞基底层(basal layer)为粗网状纹路，上层中心处有一长中心刺(central spine)向上立起，中心刺所在位置等长向外辐射三根中心脊(central ridges)，中心脊末端有三根直立的次长外围刺(sub-peripheral spines)；另一种体鳞有三层结构，其中基底层为致密网状纹，并有三个次中心孔(sub-peripheral pores)，中层和顶层各有一三叉中心脊。该研究改变了异帽藻分类研究中一种异帽藻对应一种体鳞结构的观点。 |
| 关键词: 异帽藻 体鳞 蛋白核 赤潮 分类学 |
| DOI：10.11693/hyhz20230400084 |
| 分类号: |
| 基金项目:国家自然科学基金（42276131, 41876139）；中央级公益性科研院所基本科研业务费专项资金项目（JG2108）；宁波市自然科学基金（20221JCGY010488） |
附件 |
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| The ultramorphological and molecular characters of hetercapasa niei (dinophyceae) from the east China sea |
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leng tian ze1, Gu hai feng2, Wang rui fang3, Guo zhuo ran1, Lu dou ding1, Dai xin feng1
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1.Second Institute of Oceanography, Ministry of Natural Resources;2.Third Institute of Oceanography, Ministry of Natural Resources;3.Ocean College, Zhejiang University
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| Abstract: |
| The genus Hetercapasa includes several toxic or harmful bloom-forming species, among which Hetercapasa niei, recently isolated from a toxic harmful algae bloom outbreak off the coastal water of Fujian Province, has been shown to be toxic. However, its ultrastructure, especially the body scale which is an important taxonomic feature of the genus Hetercapasa, has not been exhaustively studied. In this study, a strain (SIO-H3) isolated from the Xiamen Bay was analyzed using various morphological methods along with phylogenetic analyses based on internal transcribed spacer (ITS) gene sequences. The results showed that the strain (SIO-H3) was Hetercapasa niei. Its cell had a biconical shape with a length of 14.3-18.2 μm and a width of 8.4-10.9 μm，with a plate formula of po, cp, 5', 3a, 7", 6c, 5s, 5', 2"". Unlike previous descriptions, cells had several pyrenoids and two different body scale. One had a rough reticulate pattern in basal layer with a central spine standing upward at the top layer center, and three central ridges radiating outward in equal length from it. There were also three sub-peripheral spines standing upward at the end of each central ridge. The other body scale had a three-layer structure with a dense reticulate pattern in the basal layer with three sub-central pores and trigeminal central ridge in both middle and top layers. This study updated the view that one species had one type of body scale structure in the taxonomic study of Heterocapsa. |
| Key words: Heterocapsa body scale pyrenoid harmful algae taxonomy |