| 摘要: |
| 为了解同一育苗厂育苗的熊本牡蛎幼体、葡萄牙牡蛎幼体和长牡蛎幼体共生菌群随宿主发育的演替是否存在差异，采用16S rRNA高通量测序技术对这3种牡蛎幼体4个发育时期的共生细菌群落构成进行了比较。结果显示，在它们的4个发育时期共鉴定到3484个细菌OTUs，其中在D形幼虫、壳顶幼虫、眼点幼虫和稚贝期共有的OTU数分别占总OTU数的 43.1%、24.9%、31.3%和36.8%。4个发育时期的优势菌主要隶属于变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）和放线菌门（Actinobacteria），而其丰度在不同发育时期存在变化，特别是在细菌属水平差异显著。在4个发育时期3种牡蛎幼体共生菌群多样性有所差异，其中在眼点幼虫期的长牡蛎幼体中菌群多样性最高，而在壳顶幼虫期的熊本牡蛎幼体中菌群多样性最低。值得注意的是，3种牡蛎幼体的共生菌群结构随宿主发育呈现相似的演替变化，且在相同发育时期菌群结构差异显著。进一步对它们各发育时期的共生菌群潜在功能进行比较，发现由共生细菌参与的糖类的生物合成和代谢、脂质代谢、免疫系统等功能通路的丰度在D形幼虫期最高；而参与氨基酸代谢、细胞生长和死亡、其他次级代谢物的生物合成等功能通路的丰度在稚贝期最高但在壳顶幼虫期最低。这些结果表明熊本牡蛎幼体、葡萄牙牡蛎幼体和长牡蛎幼体4个发育时期的共生细菌群落构成存在较大差异，可能受宿主遗传的影响明显。 |
| 关键词: 牡蛎幼体 共生菌群 发育时期 构成特征 演替变化 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金 |
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| Analysis on the successional difference of structure and composition of commensal bacterial communities in the larvae of Crassostrea sikamea, Crassostrea angulata and Crassostrea gigas with host development |
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DAI Wen-Fang1, YE Jing2, LIU Sheng1, LIN Zhi-Hua1, XUE Qing-Gang1
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1.Ninghai Marine Biological Seed Industry Research Institute,Zhejiang Wanli University;2.College of Biological and Environmental Sciences, Zhejiang Wanli University
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| Abstract: |
| To understand whether there exist differences in the succession of commensal bacterial communities in the larvae of Crassostrea sikamea, Crassostrea angulata and Crassostrea gigas with host development, the structure and composition of commensal bacterial communities among three oyster larvae at four developmental stages were compared by 16S rRNA high-throughput sequencing technology. The results showed that a total of 3,484 bacterial OTUs were identified in the four developmental stages of three oyster larvae, among which the numbers of shared OTUs in the D-shaped, veliger, pediveliger and spat stages accounted for 43.1%, 24.9%, 31.3% and 36.8% of total OTUs, respectively. The dominant bacteria at four developmental stages were attached to Proteobacteria, Bacteroidetes and Actinobacteria, and their abundance varied in different larval developmental stages. In particular, significant differences were observed at the bacterial genus level. The diversity of commensal bacterial communities of three oyster larvae was different in four developmental stages. The bacterial diversity was the highest in C. gigas larvae at pediveliger stage, but was the lowest in C. sikamea larvae at veliger stage. Notably, the structure of commensal bacterial communities among three oyster larvae showed similar succession change with host development, and the significant variation was found at same development stage. Further, comparison of the potential functions of its commensal bacterial communities at different developmental stages showed that the abundances of functional pathways involved in the carbohydrate biosynthesis and metabolism, lipid metabolism and immune system were the highest at the D-shaped stage. Whereas the abundances of functional pathways involved in the amino acid metabolism, cell growth and death, and biosynthesis of other secondary metabolites were the highest at the spat stage, but were the lowest at veliger stage. These results indicated that there were significant differences in the structure and composition of commensal bacterial communities in the larvae of C. sikamea, C. angulata and C. gigas at four developmental stages, which may be obviously influenced by host genetics. |
| Key words: oyster larvae commensal bacterial community development stage structural and compositional characteristics successive change |