|
|
| |
|
|
| 本文已被：浏览 3次 下载 0次 |
|
|
|
| 金虎杂交斑三倍体、二倍体生长、发育比较及形态性状差异分析[*] |
|
邓远1,2, 苏金枝1,2, 李振通1,3, 黎琳琳1,3, 邱弈树1,4, 丁小雨5,4, 段鹏飞5, 王心怡5, 王林娜1,3, 刘阳1,3, 马文辉6, 赵侠6, 李胜忠4, 田永胜5,3
|
|
1.农业农村部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 2.新疆农业大学生命科学;3.中国水产科学研究院海南创新研究院;4.新疆农业大学生命科学学院;5.农业农村部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所;6.莱州明波水产有限公司
|
|
| 摘要: |
| 为了解金虎杂交斑三倍体的生长与形态特征，本研究利用金虎杂交斑三倍体（JH-3N）和二倍体群体（JH-2N），通过生长对比养殖分析了2种不同倍性杂交石斑鱼的生长性能，同时采用多元统计分析方法对JH-3N、JH-2N及母本棕点石斑鱼（EF）和父本蓝身大斑石斑鱼（ET）的形态特征进行统计分析。结果显示，在90 d生长周期内，JH-3N的体质量较JH-2N提高9.59%。对性腺组织学观察表明，金虎杂交斑三倍体卵巢发育较二倍体滞后。经卡方检验显示，JH-3N与JH-2N及其父母本的7个可数性状都没有显著性差异。方差分析多重比较显示，4个石斑鱼群体除Q（臀鳍前端基部至尾鳍长）外，其它18个比例性状差异显著（p<0.05）。聚类分析显示，JH-3N为单独一支，与其它3个群体分离。主成分分析共得到累计贡献率达63.273%的3主成分，主要体现了鱼体的背腹轴形态变量。判别分析显示，对JH-3N群体的判别准确率为100%，对4个群体的综合判别率达100%。研究结果表明，JH-3N具有生长速度更快、性腺发育滞后的优良性状，表型性状与JH-2N具有显著性差异，具备在生产上推广应用的前景，研究结果对金虎杂交斑杂交倍性育种及种群鉴定提供了丰富的生物学基础数据。 |
| 关键词: 金虎杂交斑 三倍体 生长速度 形态特征 多元统计 |
| DOI： |
| 分类号: |
| 基金项目:“十四五”国家重点研发计划, 2022YFD2400502号; 泰山产业领军人才项目, LJNY202109号; 山东省重点研发计划项目, 2022LZGC016号; 海南省重点研发项目, ZDYF2022XDNY243号; 财政部和农业农村部:国家现代农业产业技术体系, CARS-47-G31号; 中央公益性科研院所基本科研业务费20603022024013号; 中国水产科学研究院科研业务费2020TD19号 |
|
| COMPARISON OF GROWTH AND DEVELOPMENT OF TRIPLOIDS AND DIPLOIDS AND ANALYSIS OF MORPHOLOGICAL TRAIT DIFFERENCES IN HYBRID GROUPER |
|
dengyuan1,2, sujinzhi1,2, lizhentong1,3, lilinlin1,3, qiuyishu1,2, dingxiaoyu1,2, duanpengfei1,4, wangxinyi1,3, wanglinna1,3, liuyang1,3, mawenhui5, zhaoxia5, lishengzhong6, tianyongsheng1,3
|
|
1.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs;2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, College of Life Science, Xinjiang Agricultural University;3.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences Hainan Innovation Research Institute, Chinese Academy of Fishery Sciences;4.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;5.Ming Bo Aquatic Co. Ltd;6.College of Life Science, Xinjiang Agricultural University
|
| Abstract: |
| The objective of this study was to gain insight into the growth and morphological characteristics of hybrid grouper triploids. To achieve this, the growth performance of two different ploidy hybrid grouper populations was analysed through a growth comparison culture using hybrid grouper triploids (JH-3N) and diploid populations. (JH-2N) and multivariate statistical analysis was also employed to statistically analyse the growth performance of JH-3N, JH-2N and the maternal Epinephelus .fuscoguttatus (EF) and paternal Epinephelus tukula (ET). The results demonstrated that the body mass of the triploid JH-3N was 9.59% higher than that of the diploid JH-2N during the 90-day growth cycle. Histological observation of the gonads revealed that the development of the ovary in the triploid JH-3N lagged behind that of the diploid. The chi-square test demonstrated that none of the seven countable traits exhibited significant differences between the JH-3N and JH-2N populations and their parents. The ANOVA multiple comparisons revealed that the four grouper populations exhibited significant (p < 0.05) differences in 18 proportional traits, with the exception of Q (anterior base of anal fin to caudal fin length). Cluster analysis demonstrated that the JH-3N population constituted a distinct unit, separate from the other three groups. Principal component analysis yielded a total of three principal components, with a cumulative contribution of 63.273%, which mainly reflected the dorso-ventral axis morphological variables of the fish. Discriminant analysis demonstrated that the discrimination accuracy for the JH-3N group was 100%, and that the combined discrimination rate for the four groups was also 100%. The results of the study demonstrated that JH-3N exhibited superior traits, including accelerated growth and delayed gonadal development. These phenotypic traits were found to be distinct from those observed in JH-2N, which holds the potential for popularisation and application in production. The study also provided a wealth of biological data, offering a foundation for hybrid ploidy breeding and population identification of hybrid grouper. |
| Key words: Hybrid grouper Triploid Growth rate Morphological characters Multivariate statistics |
|
|
|
|
|
|