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| 大鳞副泥鳅台湾种群(Paramisgurns dabryanus ssp. Taiwan)及选育品系“赣红1号”(“Ganhong No.1”)体色早期发育和相关基因的表达分析* |
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胡艳1,2,3, 刘毅4, 马爱军①5, 王海华6, 孙志宾5, 蒋宇航5, 曹郡文5, 张皓5, 黄智慧①5, 马本贺6
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1. 江西师范大学生命科学学院 江西南昌 330022;2. 海水养殖生物育种与可持续产出全国重点实验室 中国水产科学研究院黄海水产研究所 中国-东盟海水养殖技术“一带一路”联合实验室(青岛) 青岛市海水鱼种子工程与生物技术重点实验室 山东青岛 266071;3. 江西省水产科学研究所 江西南昌 330039;4.江西师范大学生命科学学院 江西南昌;5.海水养殖生物育种与可持续产出全国重点实验室 中国水产科学研究院黄海水产研究所 中国-东盟海水养殖技术“一带一路”联合实验室青岛 青岛市海水鱼种子工程与生物技术重点实验室 山东青岛;6.江西省水产科学研究所 江西南昌
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| 摘要: |
| 为了探究大鳞副泥鳅台湾种群(Paramisgurns dabryanus ssp. Taiwan, 以下简称台鳅)和突变品系“赣红1号”(“Ganhong No.1”, 以下简称红鳅)体色发育形成过程中色素相关基因和通路的动态变化对体色发育及调控的影响,研究红鳅体色突变的形成机制,实验通过显微观察和荧光定量PCR等方法,探究了台鳅和红鳅成鱼五种鱼鳍色素细胞类型、早期体色发育的过程,以及不同发育时期14个体色相关基因(tyr、mitfa、foxd3、oca2、dct、tbx2a、kita、slc45a2、pmela、asip1、edn3、creb3l2、gch1和apoeb)的相对表达情况。结果表明,台鳅五种鱼鳍含有黑色素细胞、黄色素细胞和红色素细胞,红鳅五种鱼鳍含有黄色素和红色素细胞两种色素细胞；台鳅和红鳅色素细胞均在出膜后形成；荧光定量结果显示在初孵仔鱼、仔鱼24 h和成鱼皮肤中,基因tyr、slc45a2在台鳅和红鳅中的表达差异不显著,在仔鱼24 h和成鱼皮肤中,基因mitfa和foxd3在台鳅和红鳅表达中差异极显著(P<0.001)。结果表明基因tyr、slc45a2不是“赣红1号”体色形成的关键突变基因,基因mitfa和foxd3可能与红鳅体色形成机制有关,研究为深入了解“赣红1号”体色突变发生分子机制奠定了基础,并为红鳅育种提供科学依据,丰富了鱼类体色变异现象多样化的遗传机制。 |
| 关键词: 大鳞副泥鳅台湾种群(Paramisgurns dabryanus ssp. Taiwan) “赣红1号”(“Ganhong No.1”) 色素细胞 体色发育 荧光定量PCR |
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| 基金项目:江西省自然科学基金资助项目, 20224BAB215039号; 江西省主要学科和技术带头人培养计划-领军人才项目, 20225BCJ22017号; 江西省现代农业产业技术体系, JXARS-10号 |
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| Early Body Colour Development and Related Genes Expression Analysis of Selected Strain “Ganhong No.1” and Paramisgurns dabryanus ssp. Taiwan |
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HU Yan1,2,3, LIU Yi4, MA Ai-Jun5, WANG Hai-Hua6, SUN Zhi-Bin5, JIANG Yu-Hang5, CAO Jun-Wen5, ZHANG Hao5, HUANG Zhi-Hui5, MA Ben-He6
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1. College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China;2. National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Qingdao), Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao 266071, China;3. Jiangxi Fisheries Research Institute, Nanchang 330039, China;4.College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China;5.National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Qingdao), Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao 266071, China;6.Jiangxi Fisheries Research Institute, Nanchang 330039, China
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| Abstract: |
| In order to explore the influence of the dynamic changes of pigment related genes and pathways in the process of body color development and regulation of Paramisgurns dabryanus ssp. Taiwan and mutant atrain “Ganhong No.1”, to study the mechanism of “Ganhong No.1” skin color, we studied types of five adult fins’ pigment cells and the process of early body color development of Paramisgurns dabryanus ssp, Taiwan and mutant atrain “Ganhong No.1”, and the relative expression of 14 chromogenic genes (tyr, mitfa, foxd3, oca2, dct, tbx2a, kita, slc45a2, pmela, asip1, edn3, creb3l2, gch1 and apoeb) at different developmental stages through microscopic observation and quantitative Real-time qPCR methods. Results: Paramisgurns dabryanus ssp. Taiwan contains melanocytes, yellow pigment cells and red pigment cells in five fins, the “Ganhong No.1”contains yellow pigment cells and red pigment cells in the five fins; the pigment cells of both fishs were formed after membrane emergence. The qPCR result shows that there was no significant difference in the genes tyr and slc45a2 between the two groups at inttial hatching, 24 hours after larvae and the skin of adult fish. while the genes mitfa and foxd3 were significantly differently at 24 hours after larvae and the skin of adult fish (P<0.001). These results illustrate that tyr and slc45a2 genes are not the key mutant genes responsible for the color formation of “Ganhong No.1”, but mitfa and foxd3 genes may be related to the mechanism of color mutation of “Ganhong No.1” and provides a scientific basis for the breeding of “Ganhong No.1”. The study enriched the genetic mechanism of the diversity of body color variation in fish. |
| Key words: Paramisgurns dabryanus ssp. Taiwan “Ganhong No.1” pigment cell ody color development qPCR |
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