| 摘要: |
| 壳色是贝类重要的经济性状且具有可遗传性。皱纹盘鲍(Haliotis discus hannai)在人工养殖中表现出丰富的壳色变异,但其分子调控机制尚不明确。本研究以绿壳和红壳皱纹盘鲍为研究对象,采集外套膜与血淋巴组织进行转录组测序及生物信息学联合分析。结果显示,红壳个体外套膜中高表达基因显著富集于几丁质代谢、生物矿化及色素合成通路；而绿壳个体相关高表达基因主要富集于先天免疫通路。加权基因共表达网络分析筛选出5个与壳色性状显著相关的关键模块：其中cyan模块与红壳外套膜性状高度正相关,其核心基因ALAS、StAR-like-3等参与色素合成与转运；darkorange2模块与绿壳外套膜性状相关,主要富集免疫应答通路。此外,通过表达谱分析鉴定出一批壳色与生物矿化形成的关键候选基因,包括CHS1_1、CHIT10、CBP2、Tyr1、Tyr2、PIF、BCO1、Mitf、FRIS1/2和FRIY等,这些基因表现出明显的组织特异性表达模式。本研究初步揭示了红壳与绿壳皱纹盘鲍壳色变异的分子机制,阐明了外套膜与血淋巴在壳色形成中的协同作用,为解析贝类壳色遗传基础及开展定向育种提供了理论依据与关键候选基因。 |
| 关键词: 皱纹盘鲍 壳色变异 外套膜 血液 转录组 WGCNA |
| DOI： |
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| 基金项目:山东省重点研发计划（农业良种工程）项目；国家重点研发计划项目；国家重点研发计划项目；福建省科技计划项目（STS计划配套项目）；泰山学者工程资助 |
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| Transcriptomic Analysis and Gene Co-expression Network Identification of Regulatory Modules Underlying Shell Color Divergence in Haliotis discus hannai |
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chenqing1, pengzhangjie2, liyongnan2, lvfujing2, zuoxiang2, wufucun3,4, gongxiaoling5, Zhang Linlin2
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1.Shanghai Ocean University;2.Institute of Oceanology Chinese Academy of Sciences;3.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences;4.Ningbo University;5.Shanghai Ocean University, Pudong New Area, Shanghai
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| Abstract: |
| Shell color is an economically important and heritable trait in mollusks, involving complex interactions among pigment synthesis, biomineralization, and immune regulation. To elucidate the molecular basis of shell color divergence in Haliotis discus hannai, mantle and hemocyte tissues from red-shelled and green-shelled individuals were subjected to transcriptome sequencing and weighted gene co-expression network analysis (WGCNA). Differential expression analysis revealed that genes highly expressed in the red-shelled mantle were significantly enriched in chitin metabolism, biomineralization, and pigment biosynthesis pathways, whereas genes upregulated in green-shelled individuals were predominantly associated with innate immune pathways. WGCNA identified five shell color–associated modules. The cyan module was strongly correlated with the red-shelled mantle phenotype, with hub genes such as ALAS and StAR-like-3 involved in pigment synthesis and transport. In contrast, the darkorange2 module was associated with the green-shelled mantle and enriched in immune-related pathways. Furthermore, multiple candidate genes related to shell structure and pigmentation, including CHS1_1, CHIT10, CBP2, Tyr1, Tyr2, PIF, BCO1, Mitf, FRIS1/2, and FRIY, exhibited distinct tissue-specific expression patterns. These findings provide new insights into the molecular mechanisms underlying shell color divergence and highlight the coordinated roles of mantle and hemocytes, offering valuable candidate genes for genetic improvement of shell color traits. |
| Key words: Haliotis discus hannai shell color variation mantle hemolymph transcriptome WGCNA |