| 摘要: |
| 为准确、高效地选育出大菱鲆(Scophthalmus maximus)抗鳗弧菌(Vibrio anguillarum)新品种,对大菱鲆进行人工感染实验,模拟鳗弧菌的自然感染过程,利用实时荧光定量PCR (qRT-PCR)技术对其皮肤、肝脏、脾脏、肾脏、心脏、肌肉、胃、肠和鳃的相关免疫因子——溶菌酶(Lysozyme)、抗菌肽(Hepcidin)、热激蛋白70 (HSP70)、热激蛋白90 (HSP90)、免疫球蛋白(IgM)、C-型凝集素(C-type lectin)、Lily-型凝集素(Lily-type lectin)、Toll样受体(TLR5M、TLR14)和血清淀粉样蛋白P (SAP)的表达量开展研究。结果显示,10个免疫相关因子在9个组织中表达谱存在组织特异性；通过分析大菱鲆多组织免疫基因表达互作关系网络,揭示了其免疫应答的空间化特征,为了解海水养殖鱼类在病原刺激环境下组织中免疫动向提供了分子基础；同时筛选出lgM、C-type lectin和Lily-type lectin为抗病群体中持续高表达或特异性激活的潜在的抗病主效基因或关键调控因子,将筛选出的关键基因或其表达量性状位点(eQTL)转化为可用于MAS的分子标记,为抗鳗弧菌大菱鲆新品种的选育提供精准、高效的遗传工具。 |
| 关键词: 鳗弧菌 大菱鲆 免疫相关因子 基因表达 组织特异性 |
| DOI： |
| 分类号: |
| 基金项目:国家重点研发计划项目, 2022YFD2400403号; 财政部和农业农村部: 国家现代农业产业技术体系, CARS-47-G01号; 山东省重点研发计划, 2024CXPT071-2号; 中国水产科学研究院基本科研业务费专项, 2023TD26号 |
|
| ANALYSIS OF TISSUE-SPECIFIC EXPRESSION OF IMMUNE-RELATED GENES IN TURBOT (SCOPHTHALMUS MAXIMUS) INFECTED WITH VIBRIO ANGUILLARUM |
|
WANG Xin-An1, MA Yue2, TENG Yan-Yan3, DONG Chang-Hong3, GOU Dong-Hui1, HUANG Zhi-Hui1, MA Ai-Jun1
|
|
1.State Key Laboratory of Mariculture Biobreeding and Sustainable Goods,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology Qingdao;2.State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology;3.Qingdao Hengxing University of Science and Technology
|
| Abstract: |
| In order to accurately select a new variety ofSScophthalmus maximusSresistant toSVibrio anguillarum, an artificial infection experiment was conducted to simulate the natural infection process. The expression levels of key immune-related genes were detected in various tissues (including skin, liver, spleen, kidney, heart, muscle, stomach, intestine, and gills) using quantitative real-time PCR (qRT-PCR). The genes analyzed included those encoding lysozyme, the antimicrobial peptide Hepcidin, heat shock protein 70 (HSP70), heat shock protein 90 (HSP90), immunoglobulin M (IgM), C-type lectin, L-type lectin, Toll-like receptors (TLR5M and TLR14), and serum amyloid P component (SAP). This study focused on investigating the expression profiles of these immune factors. The results showed that the expression profiles of the 10 immune-related factors were tissue-specific across the nine tissues examined. Our analysis of the immune gene interaction network in multiple turbot tissues revealed the spatial characteristics of its immune response, providing a molecular basis for understanding immune trends in tissues of marine-cultured fish under pathogen challenge. Furthermore, IgM, C-type lectin, and Lily-type lectin were identified as potential key disease-resistance genes or regulatory factors, evidenced by their sustained high expression or specific activation in the disease-resistant population. The screened key genes and their expression quantitative trait loci (eQTL) were converted into molecular markers for marker-assisted selection (MAS), providing precise and efficient genetic tools for breeding new varieties ofSVibrio anguillarum-resistant turbot. |
| Key words: Vibrio anguillarum turbot Scophthalmus maximus immune-related genes gene expression tissue-specific |