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抗冻蛋白基因重组质粒的转化与扩增
徐权汉1, 钱 标2
1.中国科学院海洋研究所;2.中国科学院生物化学研究所
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TRANSFORMATION AND AMPLIFICATION OF RECOMBINANT PLASMID CONTAINING ANTIFREEZE PROTEIN GENES
Abstract:
Some of the plasmid containing antifreeze protein genes were assayed by agarose gel elec-trcphoresis and others transformed into the E.coli C600. It was amplificated in the BL-med-iam and then purified by speed-extraction method. The plasmid DNA extracted was charact-eristed by agarose gel eleetphoresis again, showing that the plasmid containing antifreeze protein genes has been transformed into the E.coli C600 and amplified.
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