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坛紫菜原生质体的超微结构观察
王素娟1, 徐志东1, 王光远2, 夏镇澳2
1.上海水产学院;2.上海植物生理研究所
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ULTRASTRUCTURAL STUDY ON PROTOPLASTS OF Porphyra haitanensis (BANGIOPHYCEAE, RHODOPHYTA)
Abstract:
The ultrastructure of protoplasts of Porphyra haitanensis (Bangiophyceae, Rhodophyta) is studied in this paper. Protoplasts of Porphyra were treated with enzyme mixture for two hours, followed by centrifugation. The enzyme mixture was composed of 1% sea snail digestive enzyme and 0.5% R-10 cellulase in 2 M glucose solution. Fluorescent microscope observation reveals that most of protoplasts obtained show no fluorescent reaction. The protoplasts were collected and fixed for the ultrastructural study. Observation under the electron microscope confirms that protoplasts have only one layer of plasma membrane surrounded without cell wall structure. The ultrastructure of protoplasts is similar to that of vegetative cells.
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