| 摘要: |
| 本文报道了王素娟等用坛紫菜(Porphyra haitanensis)营养细胞在苗绳上代替壳抱子采苗,并将幼苗下海养殖的实验。实验中,作者先酶解种藻成单离细胞,使附着于网绳上,在室内培养成 O.2—0.5mm的幼苗,然后下海养殖,1个月后,幼苗长成了20—40cm的紫菜,藻体最长者可达58cm。结果表明:冷藏一年的2—5cm的幼苗解 离细胞可以长成正常的商品紫菜。坛紫菜营养细胞可用作新苗源,它可以改革传统的生产程序,这在紫菜生产领域内具有重要意义。 |
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| A STUDY ON THE CULTIVATION OF THE VEGETATIVE CELLS AND PROTOPLASTS OF PORPHYRA HAITANENS1S I. THE CULTIVATION OF THE YOUNG BUDS ISOLATED VEGETATIVE CELLS IN SEA |
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| Abstract: |
| The conventional cultivation of Porphyra is accomplished by growing filaments in clam shells. This is followed by production of conchospores after culturing in pools of sea Water in the laboratory for five to six months. When the conchospores are fully mature, they are released and are collected onto the rope nets which are then cultured in the sea. This is not only a complex procedure but also a time consuming, economically unfeasible process. Many attempts has been made in order to improve this process. Wang et als. first isolated and culture the vegetative cells from the thalli of P. haitanensis and cultured them into young thalli with the length of 6cm in the laboratory. Trying to collectethe vegetative cells directly, instead of conchospores, and culture them in the sea, the authors carried out the experiment from September, 1984 through January, 1985. This research deals mainly with the use of the vegetative cells of P. haitanensis as seeds and their culture in the sea. The experiment process is as follows：the vegetative cells, enzymatically isolated, were attached to the rope nets and cultured in the laboratory for a month until they become buds of about 0.2-0.5 cm in length. Then the seedings were moved to the open sea for culturing. One month after culturing, the thalli could reach a maximum length of 50 cm while the average size was 20-30 cm. Their attachment density was 7-15 buds per cm of the rope. The thallus tended to grow faster as the water temperature droppfrom 21-27 c. It was proven that the vegetative cells, separated from a small thallus about 5 cm long, could grow up into a normal thallus, after being kept frozen for a year. The present study also deals with factors effecting the attachment and germintion of the vegetative cells. The result of this study shows that the vegetative cells can be used as a new seed resource, simplifying the production process of the laver seedling. The culture of P. haitanensis by this cell techniqe is a significant advancement in the field of maricultural science. |
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