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HPLC柱前衍生法测定海水中溶解态游离氨基酸
王玉君1, 李烈英1, 陆田生1
中国科学院海洋研究所
摘要:
本文采用反相高效液相色谱法测定海水中溶解态游离氨基酸,整个分析过程只需40 min就可使所有海水样品中的氨基酸得到很好分离,对各含6pmol的16种氨基酸混合样分析偏差在10%左右,检测极限为100fmo1,线性范围在1pmol到Inmol之间。
关键词:  游离氨基酸  反相高效液相色谱法  邻二苯二甲醛
DOI：
分类号:
基金项目:
HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATIONOF DISSOVED FREE AMINO ACIDS BY PRECOLUMNDERIVATIZATION IN SEAWATER
Abstract:
This paper describes a method of reversephase high performance liquid chromatohraphy for the determination of dissolved free amino acids in seawater. The whole process takes only 40 minutes to separate the amino-acids in all the sea-water samples. The coefficient of variation of 16 kinds of amino-acids samples each containing 6 p mol is about 10%, the detection is 100 f mol, the linear range is 1p mol to 1n mol.
Key words:  Free amino acids  Reversed-phase high performance liquid chromatograhy (RP-HPLC)  Ophthaldialdehyde (OPA)  
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