| 摘要: |
| 通过气相色谱方法对南极冰藻的脂肪酸进行了分析,发现环境因素对2种绿藻总脂含量有一定的影响 :(1)温度对Pyramidomonas sp.总脂含量影响不大 ,一般为8.3 %～8.9 % ,ChlorophyceaeL -4在2℃时的总脂含量最高为10.33 % ;(2)光强显著地影响这2种绿藻脂肪在细胞内的积累。随着光强由限制生长的39lx增加到3900lx ,2种绿藻的脂肪酸含量降低 ;(3)盐度的提高有利于2种绿藻脂肪的积累 ;(4)营养盐的限制也利于2种绿藻脂肪的积累。在氮源缺乏条件下 ,Pyramidomonas sp.和ChlorophyceaeL -4细胞内脂肪大量积累 ,含量分别是以NH4Cl为氮源的2.2和3.2倍。环境因素对2种绿藻脂肪酸组成和含量的影响不同 ,其脂肪含量的改变反映了南极冰藻生长的改变。环境因子能够影响这2种绿藻的脂肪含量 ,同样也影响到它们的生长。在适合南极冰藻生长的条件下 ,脂肪酸积累降低 ;反之 ,在南极冰藻生长受到限制的条件下 ,脂肪合成增加。这表明2种绿藻的脂肪含量随生存环境的变化而变化。环境因子同样影响到2种绿藻的脂肪酸组成 ,尤其是不饱和脂肪酸的组成和含量 ,但组成和其生长不存在相关性。环境因子 (温度、光强、盐度和营养盐 )对2种绿藻脂肪酸的测定结果表明 ,在2种绿藻中 ,多不饱和脂肪酸的含量高于单不饱和脂肪酸和饱和脂肪酸。因此，这2 种绿藻可望为多不饱和脂肪酸的开发利用提供藻种资源。 |
| 关键词: 南极冰藻 绿藻 脂肪酸 环境因子 |
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| 基金项目:国家自然科学基金资助项目(40206022,40406003) |
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| The influence of environment factors on lipid content and fatty acid composition in two species of Antarctic green microalga |
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| Abstract: |
| Effect of environment factors (temperature, irradiance, salinity and nutrient) on the lipid content and fatty acid composition of two species of Antarctic green microalga were investigated. The results showed that temperature had little effect on the lipid content of Pyramidomonas sp. while it had notable effect on Chlorophyceae L - 4, and the highest content of lipid was 10.33% under the temperature of 2℃. The lowest saturation of fatty acid of two green microalgae was reached at 2℃. With irradiance decreasing from 3 900 lx to 39 lx, the lipid content of two green microalgae increased. Regarding to the fatty acid, fatty acid saturation of Pyramidomonas sp. decreased and Chlorophyceae L - 4 increased when irradiance decreased. Salinity promotion and nutrient limitation favors the lipid accumulation. The PUFA content of two green microalga decreased with the salinity decreasing. And PUFA content of two green microalga increased at no nitrogen. |
| Key words: Antarctic ice alga green alga fatty acid environmental factor |
