| 摘要: |
| 分别用含1.6×109～2.0×109个/kg的产蛋白酶益生菌Y2 -2,Y1 -13,Y2 -2和Y1 -13的混合菌剂及枯草芽孢杆菌饲料投喂4月龄的牙鲆幼鱼30d ,检测的结果 :(1)体质量增长实验中,Y2 -2组体质量增长最大 ,为19.16g±0.38g(P<0.05) ,体质量增长率最高为153.16 % ;Y1 -13组与对照组差异不显著;(2)在盲囊和小肠后段 ,Y2 -2组蛋白酶活性分别为81.52U±3.10U(P<0.05)和63.20U±5.99U(P<0.05) ,酶活性增加率分别为10.8 %和21.1 %;在小肠前段和后段 ,Y2 -2组脂肪酶活性分别为255.06U±18.84U(P<0.05)和243.30U±15.00U(P<0.05) ,酶活性增加率为27.8 %和28.2 %;在小肠前段 ,枯草芽孢杆菌组淀粉酶活性为23.49U±0.96U(P<0.05) ,酶活性增加率为12.8 %;(3)Y2 -2组的肠道产蛋白酶菌数最高为6.87±0.37(P<0.05) ,增加52.5倍 ;Y1 -13组的乳酸菌数最高为3.74±0.36(P<0.05) ,增加7.6倍 ,其次为混合菌剂组 ;Y1 -13组有抑制弧菌作用 ,弧菌量降为3.91±0.37(P<0.05),抑制率为89 % ;实验组的肠道好养性异养菌数均与对照组差异不显著(P>0.05)。 |
| 关键词: 产蛋白酶菌 牙鲆幼鱼 促进作用 |
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| 基金项目:辽宁省自然科学资金资助项目(200102067) |
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| Growth promotion by proteinase-produced-bacteria from gastrointestinal tract of flounder to flounder juveniles |
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| Abstract: |
| To increase the production of founder juveniles, we raised flounder juveniles of four - months - old for 30 days with basal feed mixed by proteinase - produced bacteria Y2 - 2，Y1 - 13, Y2 - 2 + Y1 - 1 and Bacillus subpilis. The paper reported growth weight, intestinal bacteria and digestive enzyme activity in digestive tract on flounder juveniles. The results showed that ( 1) the Y2 - 2 group had the most weight increase, which was 19.16 g ± 0.38 g(P < 0.05) by 153.16% increasing. ( 2) the digestive protease activity of Y2 - 2 group were 81.52 U ± 3.10 U(P < 0.05) and 63.20 U ± 5.99 U(P < 0.05)at diverticulum pyloricum and hindgut, and its increase rate was 10.8% and 21.1% respectively. The lipase activity of the Y2 - 2 group was 255.06 U ± 18. 84 U(P < 0.05) and 243.30 U ± 15.00 U(P < 0.05) at foregut and hindgut, its increase rate were 27.8% and 28.2% respectively. The amylase activity of Bacillus subpilis group was 23.49 U ± 0.96 U(P < 0.05) at foregut，and its increase rate was 12.8%. ( 3 ) the number of proteinase - produced bacteria on digestive tract of Y2 - 2 group was the most, which was 6.87 ± 0.37(P < 0.05) , and increased 52.5 times; the number of Lactobacillus on digestive tract was the most, which was 3.74 ± 0.36(P < 0.05), and increased 7.6 times; the number of Vibrio spp. on digestive tract of Y1 - 13 group dropped to 3.91 ± 0.37(P < 0.05) , the inhitory rate was 89; total bacteria counts slightly increased. |
| Key words: bacteria produced proteinase flounder juveniles growth promotion |
