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不同保存条件下中华哲水蚤基因组DNA提取的比较
方旅平1, 林元烧1, 曹文清1
厦门大学海洋与环境学院
摘要:
以采自厦门港的中华哲水蚤 (Calanus sinicus)为对象 ,研究多种保存条件对中华哲水蚤基因组DNA提取的影响。结果表明 ,除5%福尔马林保存的样品没有提取到DNA之外 ,其余样品均能成功提取出基因组DNA ;以该DNA为模板通过相应引物成功扩增出线粒体DNACOI基因片段。其中无水乙醇保存样品提取可靠的基因组DNA方法的建立 ,为野外样品保存工作提供了一个简便 ,经济的途径。
关键词:  中华哲水蚤(Calanus sinicus)  基因组DNA提取  保存方法
DOI：
分类号:
基金项目:国家自然科学基础研究重大课题资助项目(G1999043708)
Genomic DNA extraction of Calanus sinicus different preser-vations
Abstract:
Extraction of genomic DNA from preserved specimen is a key step to study phylogeny and molecular genetics of zooplankton. This paper compared the effect of different preservations on extraction of genomic DNA adopted a small crustacean copepod, Calanus sinicus, collected from Xiamen waters. Except the specimen fixed in 5% of formalin solution, genomic DNA was well extracted by using the specimen fixed in 95% ethanol and placed at 4 ℃, 100% ethanol at 4 ℃ and TE solution at - 80 ℃. The genomic DNA can be used as a template for PCR process of mtCOI gene fragment.
Key words:  Calanus sinicus  genomic DNA extraction  preservation
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