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褐藻酸降解酶的制备及其性质研究
袁兆慧1, 韩丽君1, 林伟1, 韩宝芹2
1.中国科学院海洋研究所;2.中国海洋大学
摘要:
通过培养褐藻酸降解菌交替单胞菌(Alteromonas　sp.)菌株H -1使其产酶,研究了该酶的性质。结果表明 ,该菌在25℃培养72h时产酶量最高。褐藻酸酶作用的最适底物质量分数为1%～2% ,最适pH值为7.5 ,最适反应温度为40℃ ,温度升高酶活力急剧下降。
关键词:  褐藻酸降解菌  褐藻酸降解酶  制备
DOI：
分类号:
基金项目:国家海洋863资助项目 (D12022509,D10022508)
Preparation of alginase and its properties
Abstract:
Alginase was prepared by culturing an alginic acid decomposing bacterium Alteromonas sp. strain H - 1.　The bacterium produced a maximum amount of alginase when it was incubated at 25 ℃ for 72h.　 The optimum alginate　sodium concentration was 1% ～ 2%, the optimum pH and temperature was 7.5 (Tris - HCl buffer) and 40 ℃ respectively.　The activity of the enzyme would reduce sharply if the temperature was above 40 ℃.
Key words:  alginic acid decomposing bacterium  alginase  preparation  property
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