| 摘要: |
| 应用4种蛋白酶:实验室保藏菌株Bacillus sp.SM98011和Bacillus sp.SM97010所产的中性蛋白酶SM98011和SM97010以及购自诺维信公司的碱性蛋白酶和风味蛋白酶分别酶解鲨鱼、牡蛎、扇贝和毛虾,分析了酶解液中蛋白质含量、氨基酸组成及肽的含量,并测定了酶解液的抗氧化能力。结果表明,酶解液中必需氨基酸含量丰富、均衡,营养价值较高;SM98011蛋白酶、SM97010蛋白酶和碱性蛋白酶的酶解液中肽的比例较高;而风味蛋白酶的酶解产物主要以游离的风味氨基 |
| 关键词: 游离氨基酸 肽 蛋白酶 抗氧化性 |
| DOI： |
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| 基金项目:国家海洋863计划资助项目(2003AA625040) |
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| Enzymatic hydrolysis of marine proteins and hydrolysates antioxidant activity |
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| Abstract: |
| Marine proteins, shark, oyster, scallop, shrimp were hydrolyzed with four proteases: SM98011 produced by Bacillus sp.; SM98011, SM97010 produced by Bacillus sp. SM97010; Alcalase, and Flavourzyme. Their content of protein, amino acids and peptides were tested. All of the hydrolystates were rich in essential amino acids. The hydrolysates of marine proteins digested with protease SM98011, SM97010 and Alcalase were enriched in peptides. Compared to other three proteases, the Flavourzyme can dissociate more flavor amino acids. Antioxidant activity of each hydrolysate was determined. The hydrolysates from protease SM98011 and protease SM97010 possess more notable antioxidant activity than other two protease hydrolysates. |
| Key words: free amino acid peptides protease antioxidant |
