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Forskolin处理真鲷胚胎条件的研究
谭训刚1,2,3,4, 杜少军3,4, 张培军1
1.中国科学院海洋研究所;2.中国科学院研究生院;3.Center of Marine Biotechnology;4.University of Maryl and Biotechnology Institute
摘要:
研究Forskolin处理真鲷胚胎的条件。对加药时期以及Forskolin浓度进行了优化,得到了处理真鲷胚胎的加药时期为受精后6h,Forskolin的浓度为0.01mmol/L和0.02mmol/L,在此条件下处理过的胚胎体节由正常的V型变成U型。该研究结果将有利于研究Hh信号对真鲷成肌因子的影响。
关键词:  Forskolin  真鲷  U-型体节  胚胎  浓度
DOI：
分类号:
基金项目:国家自然科学基金国际合作交流项目(2003);国家自然科学基金(30270692)
The study on the condition of treatment on the embryos of sea bream by Forskolin
Abstract:
The condition that affects on the result of treatment on the sea bream embryos by Forskolin was studied. From the study, the concentration of the Forskolin and the stage that embryos were incubated in Forskolin was got. The concentration of Forskolin was 0.01 mmol/L and 0.02mmol/L; while the stage was 6 hour post fertilization at room temperature. In this condition the embryos were treatment showed a phenotype-U type somites. This study will contribute to the study on the evvect of Hh signals on myogensis in sea bream.
Key words:  forskolin  seabream  U-type somites  embryos  concentration
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