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4种石斑鱼染色体核型研究
郑莲1, 刘楚吾1, 李长玲1
湛江海洋大学水产学院
摘要:
以蜂巢石斑鱼(Epinephelus merra)、鲑点石斑鱼(Epinephelus fario)、点带石斑鱼(Epinephelus malabaricus)及黑边石斑鱼(Epinephelus fasciatus)为材料,胸腔注射PHA及秋水仙素溶液,取头肾细胞经空气干燥法制片。经核型分析,蜂巢石斑鱼与鲑点石斑鱼核型一样,2n=48=4m+6sm+4st+34t,NF=62;点带石斑鱼与黑边石斑鱼的核型一样,2n=48=48t,NF=48。由染
关键词:  石斑鱼(Epinephelus Bloch)  植物血细胞凝集素(PHA)  核型
DOI:
分类号:
基金项目:广东省重大科技兴海项目(A200099A01)
Studies on the karyotype of 4 groupers
Abstract:
The karyotypes of 4 wild groupers were examined in renal tissues by using PHA-injection air drying emthod. Their karyotype formulae are as follows: 2n=48=4m+6sm+4st+34t, NF=62 for Epinephelus merra and Epinephelus Fario; 2n=48=48t, NF=48 for Epinephelus malabaricus and Epinephelus fasciatus. According to the diploid number of 48 chromosomes, these 4 wild grouper species belong to high level of system classification. According to karyotypes difference, the groupers can be divided into two groups: one is E. merra and E. fario, the other is E. malabaricus and E. fasciatus. In addition, according to fundamental arm number (NF), E. merra and E. fario belong to specialized classification, while E. malabaricus and E. fasciatus belong to primitive classification.
Key words:  Epinephelus Bloch  PHA  karyotype
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