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几种紫菜种质资源遗传多样性的RAPD分析
陈骁1, 左正宏1, 姚继承1, 陈奕欣1
厦门大学生命科学学院
摘要:
用随机多态DNA(RAPD)标记方法对11个紫菜(Porphyra)样品进行遗传多样性检测,从46个随机引物中筛选出27个有效引物,共扩增出282条DNA带,其中多态位点224个,占79.4%。用类间平均链锁法对各样品间的遗传距离进行聚类分析。结果显示,2个条斑紫菜之间的亲缘关系很近,遗传相似系数达98.1%,和坛紫菜的亲缘关系较远;坛紫菜不同样品间的遗传相似系数在75.8%~90.6%之间,表明坛紫菜种质资源具有丰富的遗传多样性,是良种选育的遗传基础。
关键词:  随机多态DNA(RAPD)  紫菜(Porphyra)  遗传多样性
DOI：
分类号:
基金项目:国家863计划项目(2002A603023);福建省重大农业科技项目(2001Z017)
Analysis of genetic diversity of Porphyra by random amplified polimorphic DNA
Abstract:
The genetic diversity of 11 Porphyra samples were confirmed by random amplified polimorphic DNA(RAPD) markers. 27 primers were screened from 46 arbitrary primers, and a total of 282 DNA bands were amplified, among them 79.4% were polymorphic. The genetic data were analyzed by means of similarity, in a dendrogram was using between groups linkage method. The result showed that a close relationship existed between two cultivears of Porphyra yezoensis and the genetic coefficient comparability was 98.1%, while the genetic diversity level in Porphyra haitanesis was relatively high.
Key words:  random amplified polimorphic DNA(RAPD)  Porphyra  genetic diversity
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