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凡纳滨对虾幼体胃蛋白酶和类胰蛋白酶活力的研究
杨奇慧1, 周歧存1, 马丽莎1, 迟淑艳1
湛江海洋大学水产学院
摘要:
以酶学分析方法测定了凡纳滨对虾(Litopenaeus vannamei Boone)状幼体(Z2)、糠虾幼体(M2)和仔虾(P1)胃蛋白酶和类胰蛋白酶活力的变化。结果表明,在凡纳滨对虾幼体发育过程中,胃蛋白酶和类胰蛋白酶活力逐渐增大,表现为Z221,各期类胰蛋白酶的活力均比同期的胃蛋白酶活力高5倍左右,在食性转换过程中,即P1酶活力比Z2酶活力和M2酶活力显著升高(P<0.05),其中各期类胰蛋白酶活力的递增幅度均大于胃蛋白酶。说明了不同发育阶段,凡纳滨对虾胃蛋白酶和类胰蛋白酶的活力变化差异显著。
关键词:  凡纳滨对虾(Litopenaeus vannamei Boone)  幼体  胃蛋白酶  类胰蛋白酶  活力
DOI：
分类号:
基金项目:
Pepsin and trypsin-like enzyme activities in larvae of Litopenaeus vannamei Boone
Abstract:
An expriment was conducted to determine the activities of pepsin and trypsin-like enzyme in the larvae of Litopenaeus vannamei Boone using enzyme analytical method. The results indicated that the activities of pepsin and trypsin-like enzyme increased with the growth of larval prawn: zoea stageLitopenaeus vannamei Boone. During feeding habits changing, the activities of pepsin and trypsin-like enzyme in the post larval stage increased the most, and the activities of trypsin-like enzyme increased higher than that of pepsin.
Key words:  Litopenaeus vannamei Boone  larval  pepsin  trypsin-like enzyme  activities
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