| 摘要: |
| 用褐藻酸钙作固定化载体,初步探讨了胶球直径、不同接种量、CaCl2溶液浓度、褐藻酸钠浓度等固定化条件对微绿球藻(Nannochloris oculata)生长及其对养殖水质的影响。结果表明,微绿球藻固定化培养的最好条件是胶球的直径为2.5mm、微藻接种量为1×106 Cell/mL, CaCl2溶液的质量分数为3%,褐藻酸钠溶液质量分数为2%。在此条件下制备的固定化藻珠, 微绿球藻生长率较高,生长周期较长。实验期间固定化微绿球藻的生物量增加了约17倍,证明了它的生理活性不会因固定化而受干扰。引入固定化的微绿球藻不但可以降低养殖水体中氨氮、亚硝酸氮等有害因子的浓度,还能提高水中溶解氧含量,使水体环境长时间处于良好的动态平衡状态。 |
| 关键词: 固定化培养 微绿球藻 养殖水质 调控 |
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| 基金项目:广东省科技攻关项目(C20328) |
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| Immobilization of Nannochloris oculata in water quality control in shrimp mariculture |
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| Abstract: |
| By using alginate-Ca as immobilized matrix, the effects of some immobilized conditions on the growth of Nannochloris oculata and cultural water qulity are discussed. The optimal immobilized conditions are 2.5 mm syringe, initial inoculation 106 cell/ mL, 3% CaCl2, and 2% alginate. Immobilized microalgae produced in these optimal conditions have high growth rates and a long growth cycle. The biomass of N. oculata increased about 17 times during the experiment. It proves that the physiological conditions of the immobilized microalgae were not interrupted by the immobilization. At the same time, immogilized microalgae did not only reduce the concentration of the harmful factors such as NH4+-N and NO2--N, but also added DOin to water which can hold beeter balance of water environment for a long time. |
| Key words: immobilization culture Nannochloris oculata control cultural water quality |
