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乙酸对两种杜氏藻生长和细胞生化组成的影响
王培磊1, 张学成1, 孟振1, 张积军2
1.中国海洋大学海洋生命学院;2.内蒙古兰太实业股份有限公司生物分公司
摘要:
研究了乙酸对两种杜氏藻(Dunaliella salinaDunaliella parva)生长、β-胡萝卜、素积累、叶绿素a合成、脂肪酸组成和蛋白质含量的影响。结果表明,对D. salina,乙酸可明显促进生长。细胞密度最大值120×104cell/mL(pH≤8.5),β-胡萝卜素最大值102mg/g(pH≤8.0),叶绿素a含量达到104 mg/g(ph≤8.5),与对照组相比均有显著差异;乙酸还可提高单不饱和脂肪酸18. 1和多不饱和脂肪酸18:2n6的含量;蛋白含量随pH升高而提高。对D. parva,乙酸对生长无明显促进作用,也不能提高β-胡萝卜素含量,但明显提高叶绿素a含量,最大值达144 mg/g (pH≤9.0),还可提高蛋白含量,达到33.5%(ph≤9.0)。
关键词:  乙酸  盐生杜氏藻(Dunaliella salina)  巴夫杜氏藻(Dunaliella parva)  生长  β-胡萝卜素
DOI:
分类号:
基金项目:国家863计划(863-819-04-10)
Effects of acetic acid on the growth and chemical composition of two species of Dunaliella
Abstract:
The effects of acetic acid on the growth and ehcmical composition of two species of Dunaliella were investigated. Acetic acid can significantly enhance the growth rate of D. salina. The maximum cell density of 120×104 cell/mL, β-carotene content of 102 mg/g and Chl a content of 104 mg/g were observed in treated groups of pH≤8.5, pH≤8.0 and pH≤8.5, respectively. Three of them significantly differed from those of the control group. Acetic acid also enhanced both the contents of monounsaturated fatty acid of 18:1 and the content of polyunsaturated fatty acid of 18:2n6. The higher the pH values, the higher the contents of protein. To D. parva, acetic acid was found neither to enhance the growth rates, nor to enhance the contents of β-carotene of the culture media, though it was found to enhance both the content of Chl a, reaching 144 mg/g (pH≤9.0), and the content of protein, reaching 335 mg/g(pH≤9.0).
Key words:  acetic acid  Dunaliella salina  Dunaliella parva  growth  β-carotene
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