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酶联免疫法在贝类麻痹性贝毒检测中的应用
张纹1, 王军1, 苏永全1
厦门大学海洋学系
摘要:
采用酶联免疫法检测菲律宾蛤仔(Ruditapes philippin)肌肉中麻痹性贝毒(Paralytic shellfish poison,PSP)含量,通过优化实验条件,规范操作程序,确立了检测流程。试验表明以标准PSP 为参照,该检测方法平均灵敏度可达2μg/kg,标准溶液测定的变异系数2.00%～7.66%,样品精密度测试的变异系数为2.82%～8.40%,平均添加回收率达85.35%,表现出快速、灵敏、可靠等特点,适于常规工作中麻痹性贝毒的快速筛选检测。
关键词:  麻痹性贝毒  酶联免疫法  快速检测  菲律宾蛤仔(Ruditapes philippin)
DOI：
分类号:
基金项目:厦门市海洋与渔业局资助项目
Enzyme - linked immunosorbent assay(ELISA) quantitative analysis for paralytic shellflsh poison (PSP) in shellflsh
Abstract:
Quantitative analysis of Paralytic Shellfish Poison (PSP) in Ruditapes philippin was carried with Enzyme-Linked Immunosorbent Assay (ELISA). It showed that: the mean detection limit was 2μg/kg, mean recovery rate was 85.35%, and coefficients of variation of 2.82%～8.40% indicated that the screening method is rapid, reliable, and sensitive.
Key words:  paralytic shellfish posion  ELISA  screening method  Ruditapes philippin
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