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荧光法测定微藻中色氨酸的含量
吴芳1, 郭卫东1, 张润1, 夏恩琴1, 周宽波1
厦门大学海洋系, 亚热带海洋研究所
摘要:
建立了微藻中色氨酸的荧光分析方法。以NaOH为水解液水解微藻,在激发波长225nm,发射波长350 nm处测定色氨酸的荧光强度。方法线性范围0～0.07 mg/L,检测限为0.003 mg/L,回收率90%～100.1%。此法快速、简便、灵敏度高。
关键词:  色氨酸  微藻  荧光法
DOI：
分类号:
基金项目:国家自然科学基金资助项目(40106007)
Fluorescence measurement of tryptophan content in microalgae
Abstract:
This paper set up a procedure of fluor escence method to measure tryptophan in microalgae. NaOH containing 0.5% soluble starch was used as hydrolytic solution. The fluorescence intensity of tryptophan was determined with excitation wavelength 225 nm and emission wavelength 350 nm. The linear range of calibration graph was 0～0.07 mg/ L. The detection limit was 0.003 mg/L. The recovery rate of sample was 90%～100.1%.
Key words:  tryptophan  microalgae  fluorescence method
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