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一种快速制备甲藻细胞PCR扩增DNA模板的方法
张炎¹, 韩笑天¹, 邹景忠¹, 周名江¹, 王广策¹
中国科学院海洋研究所海洋生态与环境重点实验室

摘要:

报道了一种快速制备PCR扩增甲藻细胞模板DNA的方法。以赤潮甲藻塔玛亚历山大藻(Alexandrium tamarense)和链状亚历山大藻(A.catenella)为目标藻,对藻液预处理后直接用于PCR扩增,获得5.8S核糖体RNA基因及其两侧的核糖体RNA基因转录单元内基因间隔区(internal transcribed spacer, ITS)序列片段,成功测序。此方法避免了复杂的DNA提取过程。

关键词: 赤潮甲藻亚历山大藻(Alexandrium) 转录单元内基因间隔区 PCR扩增

DOI：

分类号:

基金项目:国家重点基础研究规划资助项目(2001CB409701)；国家自然科学基金资助项目(40376040号)

A method for quickly preparing PCR amplification of dinoflagellate total DNA template

Abstract:

A method for quickly preparing PCR amplification of dinoflagellate total DNA template was reported. Alexandrium tamarense and A. catenella were solved in advance and used as template of PCR amplification. The production of internal transcribed spacer including 5.8s RNA gene was sequenced. It was demonstrated that the method was simple and useful to molecular analysis of dinoflagellate.

Key words: dinoflagellate Alexandrium internal transcribed spacer PCR

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