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60Co－γ辐照对坛紫菜原生质体诱变的研究
姚继承1, 王 诚1, 左正宏1, 林 芳1, 陈奕欣1
厦门大学 生命科学学院
摘要:
利用60Co-γ射线对坛紫菜(Porphyra haitanensis)原生质体进行辐照诱变,发现原生质体对60Co-γ射线有极强的耐受性。前期的培养结果表明，原生质体是一种理想的诱变材料。诱变结果表明，60Co-γ射线对坛紫菜可诱变剂量范围很大，从200～1 300 Gy 均有突变株生成。诱变后细胞的存活率不宜作为坛紫菜诱变最佳剂量的指标。
关键词:  坛紫菜(Porphyra haitanensis)  原生质体  60Co-γ射线  诱变
DOI：
分类号:
基金项目:国家863 计划项目（2002AA603023）；福建省重大农业科技项目（2001Z017）
Mutagenesis on protoplast of Porphyra haitanensis by 60Co-γ
Abstract:
60Co-γ ray was used to induce mutant on protoplast of Porphyra haitanensis. The results showed that the protoplasts of P. haitanensis could survive under a wide range of γ-rays. Primary culture showed that the protoplast was suitable for mutagenesis. Further results also showed that mutants could be induced in a very wide range of dosages of 60Co-γ ray. The mortality was not a suitable parameter for the evaluation of the mutant-induction efficiency in P. haitanensis.
Key words:  Porphyra haitanensis  protoplast  60Co –γ ray  mutagenesis
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