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斜带髭鲷养殖群体遗传多样性RAPD分析
梁君荣1, 王军2, 苏永全2, 丁少雄2
1.厦门大学生命科学学院;2.厦门大学海洋与环境学院
摘要:
采用随机扩增多态性DNA(RAPD)技术对广东饶平海水网箱养殖的斜带髭鲷(Hapalogenys nitens Richardson)人工苗养殖群体DNA多样性进行检测。首先从40个引物(S461～S480,S501～S520)中快速筛选出32个引物,它们均能扩增出清晰稳定的DNA片段。再将该32个引物对46尾斜带髭鲷进行RAPD分析,共检测到177个位点,其中多态位点55个(分别由18个引物获得),占31.07%。由此得出该群体的平均多态性为31.07%,平均遗传差异度为0.089,表明该养殖群体遗传多样性水平较高。
关键词:  斜带髭鲷(Hapalogenys nitens Richardson)  遗传多样性  随机扩增多态性DNA(RAPD)
DOI：
分类号:
基金项目:国家自然科学基金资助项目( 30070595) ; 福建省自然科学基金资助项目( B80010003)
RAPD analysis of genetic diversity in reared Hapalogenys nitens stock
Abstract:
Genetic diversity of DNA in reared Hapalogenys nitens stocks, which were collected from Zhelin Bay in Guangdong Province in Jun. 1999, was analyzed by random amplified polymorphic DNA ( RAPD) technique in this paper. There were 177 gene loci detected from 32 primers, and fifty-five loci of them were polymorphic. So the polymorphism and the mean difference of the reared H. nitens stocks were 31.07% and 0.089 respectively. The r esults showed that the reared H. nitens population still retained richer genetic diversity.
Key words:  Hapalogenys nitens  RAPD  genetic diversity  random amplified polymorphic DNA( RAPD)
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