| 摘要: |
| 采用均匀设计,研究了硝酸钠(NO3-N)、尿素(NH2-N)和硫酸铵(NH4-N)3种氮源对亚心形扁藻(Platymonas subcordiformis)与球等鞭金藻(Isochrysis galbana)共培养和单种培养生长的影响。结果表明,不同氮源对扁藻与金藻在不同培养方式下的生长均存在显著差异(P<0.05)。共培养时,硝酸钠质量浓度对扁藻和金藻的总细胞密度(Yt
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| 关键词: 氮源 亚心形扁藻(Platymonas subcordiformis) 球等鞭金藻(Isochrysis galbana) 共培养 单种培养 生长 |
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| 基金项目:浙江省教育厅资助项目(20010233);宁波市重点博士基金资助项目(02J20101-16);宁波市科技局资助项目(01N40100-58) |
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| Effects of mixed nitrogen sources on growth of mixed culture and monoculture of Platymonas subcordiformis and Isochrysis galbana |
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| Abstract: |
| Uniform design was performed to examine effects of 3 mixed nitrogen sources on growths of mixed cultures and monocultures of Platymonas subcordiformis and Isochrysis galbana. The results showed the growth performances of P. subcordiformis and I. galbana in different treatments were significantly different（P<0.05）. In mixed culture, the experimental concentration of NaNO3 had no effects on total cell density(Yt) and volumetric ratio(Yr), and the concentration of CO(NH2)2 had a positive influence upon Yt, but the concentration of(NH4)2SO4 resulted in a negative effect on Yt compared to the Yr, which was sharply increased. When the cell volmetric ratio(Xv) of inoculative P. subcordiformis to I. galbana was 14.15 and each concentration of NH2-N and NH4-N was 21.01 mg/L, the optimum Yt and Yr got to 1 508.9×104 cell/mL and 87.46, respectively. In monoculture of I. galbana, the nitrogen source of NaNO3 was slightly better than that of CO(NH2)2, and both all had positive effects on growth of I. galbana. On the contrary, nitrogen source of(NH4)2SO4 was of inverse influence on the algal growth. According to the optimum result, the maximal cell density of I. galbana was 862.6×104 cell/mL when concentrations of NO3-N and NH2-N were given at 21.01 mg/L each. Meanwhile, in monoculture of P. subcordiformis, the contrary conclusions were also drawn while enriched the same nitrogen sources and concentrations in the monoculture medium. If the inoculative cell density and concentration of NH4-N were 4.6×104 cell/mL and 21.01 mg/L, respectively, the top cell density of P. subcordiformis can be got theoretically, which was 102.2×104 cell/mL. |
| Key words: nitrogen source Platymonas subcordiformis Isochrysis galbana mixed culture monoculture growth |
