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海蒿子多糖DEI、DEII组分的分离纯化及单糖组成分析
赵 宇1, 李俊卿1, 张立新1, 陈 悦1, 李志萍1, 陆懋荪1, 尹佩玉1
青岛大学 化工学院
摘要:
海蒿子(Sargassum confusum Agardh)经热水抽提、乙醇沉淀、DEAE-SephadexA-25柱层析分离,得到多糖DEI、DEII,糖质量分数分别为52.40%,38.80%。经酸降解、薄层层析、气相色谱-质谱分析,证明其单糖组成均为岩藻糖、木糖、半乳糖、果糖、甘露糖、葡萄糖。其中岩藻糖质量分数最高(DEI:39.3%; DEII:35.2%)。
关键词:  海蒿子(Sargassum confusum Agardh)  多糖  分离  纯化  单糖组成
DOI：
分类号:
基金项目:青岛市科技局资助项目(02-1-kchhh-42-2)
The studies on the isolation, purification, and chemical composition analysis of polysaccharides DEI and DEII from Sargassum confusum
Abstract:
The characteristics of the polysaccharide DEI and polysaccharide DEII isolated from Sargassum confusum through water extraction, ethanol precipitation and DEAE-Sephadex A-25 column chromatography sequentially were analyzed by TLC, gel electrophoresis and GC-MS. It was found that these polysaccharides were composed of fucose, xylose, galactose, fructose, mannose and glucose, among them fucose being the primarycomponent (39.3% in DEI and 35.2% in DEII).
Key words:  Sargassum confusum  polysaccharide  isolation  purification  chemical composition
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