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臭氧化海水对于弧菌DNA的损伤作用
宫小明1,2, 孙 军1, 刘 萍2, 刘 琪2, 李 健2
1.潍坊出入境检验检疫局;2.中国水产科学院黄海水产研究所
摘要:
用琼脂糖电泳法分别检测了臭氧化海水对溶藻胶弧菌(Vibrio alginolyticus)、副溶血弧菌(Vibrio parahaemolyticus)、飘浮弧菌(Vibrio natriegen)菌体内DNA和经碱性裂解法抽提后得到的裸露DNA的损伤作用。结果表明,臭氧化海水对DNA有很强的损伤和降解作用,在电泳图谱上表现为DNA带的加宽和弥散;臭氧化海水对裸露DNA的损伤作用,在电泳图谱上表现为DNA带荧光强度的增强和出现长长的拖尾。
关键词:  臭氧  弧菌属(Vibrio)  DNA
DOI：
分类号:
基金项目:国家科技攻关项目(2004BA526B02);山东省科技发展计划项目(2003-4-1)
Damage of ozone-produced oxidant to Vibrio DNA
Abstract:
The damage of ozonated seawater to DNA in vivo and in vitro is investigated by the method of agarose gel electrophoresis. The results indicate that ozoned seawater can damnify and degrade Vibrio DNA greatly. Seen from the electrophoresis pattern, DNA breadth becomes boardened and dispersion. The damage of ozoned seawater to exposed DNA showing in the electrophoresis pattern is reflected by fluorescent intensification and long tail.
Key words:  ozone  Vibrio  DNA
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